Detailed information of TA system TA192535

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 106845..107271
• Replicon: plasmid unnamed
• Accession: NZ_CP069991
• Organism: Escherichia coli strain FDAARGOS_1304

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: I6K34_RS25645
• Protein ID: WP_001372321.1
• Coordinates: 106845..106970 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	107047..107271 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K34_RS25620 (102241)	102241..102930	-	690	WP_033550311.1	conjugal transfer transcriptional regulator TraJ	-
I6K34_RS25625 (103117)	103117..103500	-	384	WP_001151538.1	conjugal transfer relaxosome DNA-binding protein TraM	-
I6K34_RS25630 (103914)	103914..104423	+	510	WP_000759173.1	transglycosylase SLT domain-containing protein	-
I6K34_RS25635 (104720)	104720..105541	-	822	WP_001234445.1	DUF932 domain-containing protein	-
I6K34_RS25640 (105651)	105651..105947	-	297	WP_001272251.1	hypothetical protein	-
I6K34_RS26220 (106247)	106247..106544	+	298	Protein_121	hypothetical protein	-
I6K34_RS25645 (106845)	106845..106970	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6K34_RS26225 (106912)	106912..107061	-	150	Protein_123	plasmid maintenance protein Mok	-
I6K34_RS25650 (107083)	107083..107262	+	180	WP_001309233.1	hypothetical protein	-
- (107047)	107047..107271	-	225	NuclAT_0	-	Antitoxin
- (107047)	107047..107271	-	225	NuclAT_0	-	Antitoxin
- (107047)	107047..107271	-	225	NuclAT_0	-	Antitoxin
- (107047)	107047..107271	-	225	NuclAT_0	-	Antitoxin
I6K34_RS25655 (107240)	107240..108002	-	763	Protein_125	plasmid SOS inhibition protein A	-
I6K34_RS25660 (107999)	107999..108433	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
I6K34_RS25665 (108502)	108502..110466	-	1965	WP_033550309.1	ParB/RepB/Spo0J family partition protein	-
I6K34_RS25670 (110527)	110527..110760	-	234	WP_033550308.1	DUF905 family protein	-
I6K34_RS25675 (110818)	110818..111345	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
I6K34_RS25680 (111647)	111647..112096	+	450	Protein_130	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..133493	133493

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T192535 WP_001372321.1 NZ_CP069991:c106970-106845 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T192535 NZ_CP092449:2606269-2606376 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGGACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT192535 NZ_CP069991:c107271-107047 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin