Detailed information of TA system TA192527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3582499..3583101
• Replicon: chromosome
• Accession: NZ_CP069990
• Organism: Escherichia coli strain FDAARGOS_1304

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6K34_RS17445
• Protein ID: WP_000897302.1
• Coordinates: 3582790..3583101 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K34_RS17440
• Protein ID: WP_000356397.1
• Coordinates: 3582499..3582789 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K34_RS17415 (3578572)	3578572..3579474	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
I6K34_RS17420 (3579471)	3579471..3580106	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K34_RS17425 (3580103)	3580103..3581032	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6K34_RS17430 (3581248)	3581248..3581466	-	219	WP_001314326.1	CopG family transcriptional regulator	-
I6K34_RS17435 (3581862)	3581862..3582140	-	279	WP_001296612.1	hypothetical protein	-
I6K34_RS17440 (3582499)	3582499..3582789	-	291	WP_000356397.1	NadS family protein	Antitoxin
I6K34_RS17445 (3582790)	3582790..3583101	-	312	WP_000897302.1	hypothetical protein	Toxin
I6K34_RS17450 (3583330)	3583330..3584238	+	909	WP_001331553.1	alpha/beta hydrolase	-
I6K34_RS17455 (3584302)	3584302..3585243	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K34_RS17460 (3585288)	3585288..3585725	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
I6K34_RS17465 (3585722)	3585722..3586594	-	873	WP_000920762.1	virulence factor BrkB family protein	-
I6K34_RS17470 (3586588)	3586588..3587187	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T192527 WP_000897302.1 NZ_CP069990:c3583101-3582790 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T192527 NZ_CP092449:873024-873431 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGCTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT192527 WP_000356397.1 NZ_CP069990:c3582789-3582499 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT192527 NZ_CP092449:872777-873043 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: S1P416