Detailed information of TA system TA192519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2554629..2555323
• Replicon: chromosome
• Accession: NZ_CP069990
• Organism: Escherichia coli strain FDAARGOS_1304

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: I6K34_RS12490
• Protein ID: WP_001263500.1
• Coordinates: 2554629..2555027 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6K34_RS12495
• Protein ID: WP_000554758.1
• Coordinates: 2555030..2555323 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K34_RS12465 (2549994)	2549994..2550452	-	459	WP_001291988.1	xanthine phosphoribosyltransferase	-
I6K34_RS12470 (2550713)	2550713..2552170	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
I6K34_RS12475 (2552227)	2552227..2552841	-	615	WP_000602123.1	peptide chain release factor H	-
I6K34_RS12480 (2552838)	2552838..2553977	-	1140	WP_000521577.1	RNA ligase RtcB family protein	-
I6K34_RS12485 (2554167)	2554167..2554619	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
I6K34_RS12490 (2554629)	2554629..2555027	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6K34_RS12495 (2555030)	2555030..2555323	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6K34_RS12500 (2555375)	2555375..2556430	-	1056	WP_001226168.1	DNA polymerase IV	-
I6K34_RS12505 (2556501)	2556501..2557286	-	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
I6K34_RS12510 (2557258)	2557258..2558970	+	1713	Protein_2472	flagellar biosynthesis protein FlhA	-
I6K34_RS12515 (2559049)	2559049..2559207	+	159	WP_014639450.1	hypothetical protein	-
I6K34_RS12520 (2559290)	2559290..2559787	-	498	WP_079437275.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	2554629..2572759	18130

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T192519 WP_001263500.1 NZ_CP069990:c2555027-2554629 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T192519 NZ_CP092447:c1845014-1844862 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT192519 WP_000554758.1 NZ_CP069990:c2555323-2555030 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT192519 NZ_CP092447:c1845638-1845039 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACTGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATATTG
AAAAGCATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATTGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCATAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3