Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1324991..1325553 Replicon chromosome
Accession NZ_CP069990
Organism Escherichia coli strain FDAARGOS_1304

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I6K34_RS06410 Protein ID WP_000605675.1
Coordinates 1324991..1325269 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6K34_RS06415 Protein ID WP_000781370.1
Coordinates 1325269..1325553 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K34_RS06385 (1320578) 1320578..1321009 - 432 WP_000152310.1 peroxiredoxin OsmC -
I6K34_RS06390 (1321354) 1321354..1321569 + 216 WP_000495766.1 biofilm-dependent modulation protein -
I6K34_RS06395 (1321671) 1321671..1321808 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6K34_RS06400 (1321964) 1321964..1323661 + 1698 WP_000433462.1 malate dehydrogenase -
I6K34_RS06405 (1323795) 1323795..1324805 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
I6K34_RS06410 (1324991) 1324991..1325269 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K34_RS06415 (1325269) 1325269..1325553 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
I6K34_RS06420 (1325782) 1325782..1326435 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
I6K34_RS06425 (1326428) 1326428..1327312 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
I6K34_RS06430 (1327325) 1327325..1330372 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T192516 WP_000605675.1 NZ_CP069990:1324991-1325269 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T192516 NZ_CP092446:485643-485993 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT192516 WP_000781370.1 NZ_CP069990:1325269-1325553 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT192516 NZ_CP092446:485356-485637 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References