Detailed information of TA system TA192502

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 81595..81834
• Replicon: plasmid unnamed2
• Accession: NZ_CP069982
• Organism: Escherichia coli strain FDAARGOS_1291

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: I6K21_RS25040
• Protein ID: WP_023144756.1
• Coordinates: 81700..81834 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	81595..81655 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K21_RS25000 (77512)	77512..77895	+	384	WP_001151566.1	conjugal transfer relaxosome DNA-binding protein TraM	-
I6K21_RS25005 (78029)	78029..78706	+	678	WP_001348626.1	PAS domain-containing protein	-
I6K21_RS25010 (78794)	78794..79021	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
I6K21_RS25015 (79055)	79055..79417	+	363	WP_000338606.1	type IV conjugative transfer system pilin TraA	-
I6K21_RS25020 (79422)	79422..79733	+	312	WP_000012107.1	type IV conjugative transfer system protein TraL	-
I6K21_RS25025 (79755)	79755..80057	+	303	Protein_108	type IV conjugative transfer system protein TraE	-
I6K21_RS25030 (80118)	80118..80815	+	698	WP_223216367.1	IS1 family transposase	-
I6K21_RS25035 (81342)	81342..81628	+	287	Protein_110	DUF2726 domain-containing protein	-
- (81595)	81595..81655	-	61	NuclAT_1	-	Antitoxin
- (81595)	81595..81655	-	61	NuclAT_1	-	Antitoxin
- (81595)	81595..81655	-	61	NuclAT_1	-	Antitoxin
- (81595)	81595..81655	-	61	NuclAT_1	-	Antitoxin
I6K21_RS25040 (81700)	81700..81834	+	135	WP_023144756.1	Hok/Gef family protein	Toxin
I6K21_RS25045 (82131)	82131..82385	+	255	WP_000083850.1	replication regulatory protein RepA	-
I6K21_RS25055 (82622)	82622..82696	+	75	WP_031943482.1	RepA leader peptide Tap	-
I6K21_RS25060 (82689)	82689..83546	+	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
I6K21_RS25065 (84486)	84486..85139	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
I6K21_RS25070 (85232)	85232..85489	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
I6K21_RS25075 (85422)	85422..85823	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
I6K21_RS25080 (86108)	86108..86248	+	141	Protein_118	DUF262 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(B) / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / dfrA14	-	1..116491	116491
	-	flank	IS/Tn	-	-	80312..80815	503
	-	flank	IS/Tn	-	-	86498..87001	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T192502 WP_023144756.1 NZ_CP069982:81700-81834 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T192502 NZ_CP092439:476205-476555 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Nucleotide

• Length: 61 bp

>AT192502 NZ_CP069982:c81655-81595 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin