Detailed information of TA system TA192499

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 73864..74133
• Replicon: plasmid unnamed2
• Accession: NZ_CP069982
• Organism: Escherichia coli strain FDAARGOS_1291

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: I6K21_RS24975
• Protein ID: WP_001372321.1
• Coordinates: 74008..74133 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	73864..73929 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K21_RS25730	68883..69158	+	276	WP_071780182.1	hypothetical protein	-
I6K21_RS25735	69399..69605	+	207	WP_000547975.1	hypothetical protein	-
I6K21_RS24945	69631..70170	+	540	WP_000290841.1	single-stranded DNA-binding protein	-
I6K21_RS24950	70233..70466	+	234	WP_000005990.1	DUF905 family protein	-
I6K21_RS24955	70532..72490	+	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
I6K21_RS24960	72545..72979	+	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
I6K21_RS24965	72976..73738	+	763	Protein_93	plasmid SOS inhibition protein A	-
I6K21_RS24970	73707..73895	-	189	WP_001299721.1	hypothetical protein	-
-	73707..73931	+	225	NuclAT_0	-	-
-	73707..73931	+	225	NuclAT_0	-	-
-	73707..73931	+	225	NuclAT_0	-	-
-	73707..73931	+	225	NuclAT_0	-	-
-	73864..73929	-	66	-	-	Antitoxin
I6K21_RS25740	73917..74066	+	150	Protein_95	plasmid maintenance protein Mok	-
I6K21_RS24975	74008..74133	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6K21_RS25745	74353..74583	+	231	WP_001426396.1	hypothetical protein	-
I6K21_RS25750	74581..74754	-	174	Protein_98	hypothetical protein	-
I6K21_RS24980	74824..75030	+	207	WP_000275859.1	hypothetical protein	-
I6K21_RS24985	75055..75342	+	288	WP_000107535.1	hypothetical protein	-
I6K21_RS24990	75461..76282	+	822	WP_001234469.1	DUF932 domain-containing protein	-
I6K21_RS24995	76579..77169	-	591	WP_001376243.1	transglycosylase SLT domain-containing protein	-
I6K21_RS25000	77512..77895	+	384	WP_001151566.1	conjugal transfer relaxosome DNA-binding protein TraM	-
I6K21_RS25005	78029..78706	+	678	WP_001348626.1	PAS domain-containing protein	-
I6K21_RS25010	78794..79021	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(B) / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / dfrA14	-	1..116491	116491

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T192499 WP_001372321.1 NZ_CP069982:74008-74133 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T192499 NZ_CP092437:c382327-381923 [Rhizobium sp. K102]
GTGATCACGCATCTTATCGACACCAATGCGGTGATCGCGCTGATCGGCAGAAAATCCGAGGCGCTGCTGAAGCGCGTGGT
CGACAGCGACGAGGGATCGATCGGGCTTTCGACTGTCGTGATGCATGAGCTCTATTATGGCGCCTATAAGAGCGCGAAAA
TCTCCCACAATCTCGAAACCCTGCGCCTGTTCATGGCGGATTTCCCCGCCGTCGGTTTCGAGCGCGAAGATGCGCTGGCA
TCGGGAGAGATCCGCGCAGCATTGGCGGCAAAGGGAACGCCGATCGGTCCCTACGATGTGCTGATCGCCGCCCAGGCGAA
GACCCGGGATCTCGTTCTCATCACGAACAATGTCGGGGAGTTCCGGCGCGTGGATGGCCTTGATGTCGAGGATTGGACGG
CCTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT192499 NZ_CP069982:c73929-73864 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin