Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4478038..4478600 Replicon chromosome
Accession NZ_CP069980
Organism Escherichia coli strain FDAARGOS_1291

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag I6K21_RS21250 Protein ID WP_000605676.1
Coordinates 4478322..4478600 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6K21_RS21245 Protein ID WP_000781370.1
Coordinates 4478038..4478322 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K21_RS21230 (4473220) 4473220..4476267 + 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -
I6K21_RS21235 (4476280) 4476280..4477164 + 885 WP_001240565.1 formate dehydrogenase N subunit beta -
I6K21_RS21240 (4477157) 4477157..4477810 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
I6K21_RS21245 (4478038) 4478038..4478322 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
I6K21_RS21250 (4478322) 4478322..4478600 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K21_RS21255 (4478786) 4478786..4479796 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
I6K21_RS21260 (4479930) 4479930..4481627 - 1698 WP_000433464.1 malate dehydrogenase -
I6K21_RS21265 (4481784) 4481784..4481921 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6K21_RS21270 (4482023) 4482023..4482238 - 216 WP_000495766.1 biofilm-dependent modulation protein -
I6K21_RS21275 (4482583) 4482583..4483014 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T192485 WP_000605676.1 NZ_CP069980:c4478600-4478322 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T192485 NZ_CP092429:706003-706449 [Mycobacterium ulcerans]
ATGGCGAAACTTTCCGGGTCGATCGACGTGCCGCTGTCTCCGGACGAGGCATGGATGCACGCATCCGATCTCTCCCGCTT
CAAGGAGTGGCTGACGATCCACCGAGTGTGGCGCAGCACGTTGCCCGAAACCCTCGAAAAGGGTGCGGTCGTCGAATCAA
TCGTGCAGGTCAAGGGCATGCACAACCGCATCAAATGGACCATCGTGCGCTACCAGCCGCCGGAAGGCATGACCCTCAAC
GGTGACGGGGTCGGCGGCGTCAAGGTCAAGCTGATGGCCAAGGTTGCAGCCGGAGAACAAGGCTCGATCGTCAGCTTCGA
CGTGCACCTTGGTGGGCCGGCTCTGTTCGGTCCGATCGGCATGGTCGTCGCCGCGGCGCTGCGCAGCGATATCAACGCAT
CGCTGCGGAACTTCGTCACCGTATTCGCCCCGCCGGCTGCCGGCTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT192485 WP_000781370.1 NZ_CP069980:c4478322-4478038 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT192485 NZ_CP092429:705810-705998 [Mycobacterium ulcerans]
ATGGGAATCCTGGACAAGGCGAAAGACCTGCTGGCGCAGAACGCCGACAAGGTCGAGACGGTGATCGACAAGGCCGGCGA
ATTCGTCGACGAGAAGACCGAAGGGAAGTACTCCGACACCATCCACAAGGTGCAAGAGGGGGCCAAGAAAGTCGCTGGTC
CCGGCGCCGCGGGCGAGCAGCAGAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References