Detailed information of TA system TA192476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3078079..3078729
• Replicon: chromosome
• Accession: NZ_CP069980
• Organism: Escherichia coli strain FDAARGOS_1291

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I6K21_RS14285
• Protein ID: WP_000263532.1
• Coordinates: 3078388..3078729 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: I6K21_RS14280
• Protein ID: WP_000212552.1
• Coordinates: 3078079..3078378 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K21_RS14255 (3073527)	3073527..3073838	-	312	WP_000415004.1	hypothetical protein	-
I6K21_RS14260 (3073843)	3073843..3074235	-	393	WP_000285322.1	flagellar export chaperone FliS	-
I6K21_RS14265 (3074258)	3074258..3075574	-	1317	WP_000609678.1	flagellar filament capping protein FliD	-
I6K21_RS14270 (3075779)	3075779..3076693	-	915	WP_000949083.1	lateral flagellin LafA	-
I6K21_RS14275 (3077179)	3077179..3078021	+	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
I6K21_RS14280 (3078079)	3078079..3078378	-	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
I6K21_RS14285 (3078388)	3078388..3078729	-	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K21_RS14290 (3078805)	3078805..3079782	-	978	WP_001195938.1	hypothetical protein	-
I6K21_RS14295 (3079799)	3079799..3080728	-	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
I6K21_RS14300 (3080743)	3080743..3082119	-	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
I6K21_RS14305 (3082295)	3082295..3082594	-	300	WP_000867281.1	rod-binding protein	-
I6K21_RS14310 (3082594)	3082594..3083694	-	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T192476 WP_000263532.1 NZ_CP069980:c3078729-3078388 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T192476 NZ_CP092419:c1312236-1311910 [Leptolyngbya boryana IU 594]
ATGGCAATGGTAGTTCGTCGTTTTGATGTGTTTCTTGTTCAGCTAGATCCAACTGTTGGATCTGAAATTCAGAAGACCCG
TCCTTGTATAATCGTCTCTCCCGATGAGATGAATCGTTTACTCAAAACGGTTTTGATAGCTCCATTAACTTCTGTAAGAC
GAAACTTCCCATTTCGAGTTGATTGTCAGTTTCAAGGCAAAGCGGGTCAGATTGTCCTAGATCAAATTCGAGCAGTAGAT
AAAACCCGACTTGTTCAATTTCTGGGTCAAATTGGCGAGGAGCAAAGAGCGCTTCTCGATACGATAGCTGACCTCTTTGC
TGCCTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT192476 WP_000212552.1 NZ_CP069980:c3078378-3078079 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT192476 NZ_CP092419:c1312487-1312224 [Leptolyngbya boryana IU 594]
ATGAGTGGAACAATTCGGACTCGACTTGTCAAATTTGGTAATTCTCAAGGAATTCGGATTCCGAAGCTGTTACTAGAGCA
AAGTGGCATTCAAAGTGAAGTTGAAATCGAAGTCGAAGGCAATCGCTTGATTATTCGTCCTGTATCTCATCCTCGTGCTG
GATGGGAAGCAGCGATCGATGCAGAAATCGCAAAATCTGGGGATGAAGGCTTGCTGGATGATGAAACCTCAACTCAATGG
GACGAAACCGAATGGCAATGGTAG

Structures

Antitoxin

• AlphaFold DB: U9YXQ7