Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1303709..1304508 Replicon chromosome
Accession NZ_CP069980
Organism Escherichia coli strain FDAARGOS_1291

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag I6K21_RS05955 Protein ID WP_000347269.1
Coordinates 1304044..1304508 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0YUB5
Locus tag I6K21_RS05950 Protein ID WP_001309780.1
Coordinates 1303709..1304044 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K21_RS05935 (1299494) 1299494..1300264 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
I6K21_RS05940 (1300280) 1300280..1301614 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
I6K21_RS05945 (1301989) 1301989..1303560 + 1572 WP_001273738.1 galactarate dehydratase -
I6K21_RS05950 (1303709) 1303709..1304044 + 336 WP_001309780.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
I6K21_RS05955 (1304044) 1304044..1304508 + 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
I6K21_RS05960 (1304563) 1304563..1305372 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
I6K21_RS05965 (1305621) 1305621..1306901 + 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
I6K21_RS05970 (1306924) 1306924..1307397 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
I6K21_RS05975 (1307408) 1307408..1308187 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
I6K21_RS05980 (1308177) 1308177..1309055 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
I6K21_RS05985 (1309073) 1309073..1309507 + 435 WP_000948818.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1294467..1304508 10041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T192466 WP_000347269.1 NZ_CP069980:1304044-1304508 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T192466 NZ_CP092417:1574631-1574915 [Endozoicomonas sp. SCSIO W0465]
ATGATTTTATGGGAAGAAGAGTCTCTGAATGATCGGGAAAAGATCTTTGAGTTTCTTTACGATTTTAATCCAGAAGCAGC
AGAAAAGACTGATCTGATCATTGAAGCGAAAGTCGAAAACTTATTAGAACAACCGCTTATGGGGGGTGAGCGTGAAGGCA
TCCGTGGCAGATTGCTGATCATTCCGGAAGTATCCATGATTGTTTCATACTGGGTGGATGGTAAAGATATTCGTGTAATG
CGAGTTCTTCACCAGAAACAAAAATTCCCAAGTGATCCGGGTTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12345.94 Da        Isoelectric Point: 4.8616

>AT192466 WP_001309780.1 NZ_CP069980:1303709-1304044 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT192466 NZ_CP092417:1574347-1574631 [Endozoicomonas sp. SCSIO W0465]
ATGGAAACAAGAATACAATTTCGAGTCGATGAGGAAACAAAACGCCTGGCTCAAAAAATGGCTGAAAGTCAGGGGCGCAC
GATCAGTGATGCATGCCGGGAGTTTACTGAGCAACTGGCGGAACAACAGCGTAAGTTATCCTCCCACGATCAATGGCTAA
CCCAACAAGTCAATCAGGCATTTGAGAAACTGGACTCTGGAGCAGCGGATTTTGTTGAACACGATAAAACCAAAGACGAG
ATGGCTAAGCGCAAAGCAAAAATTCGCAATAGAGGCAGCCATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB V0YUB5

References