Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1257486..1258213 Replicon chromosome
Accession NZ_CP069980
Organism Escherichia coli strain FDAARGOS_1291

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag I6K21_RS05715 Protein ID WP_000550189.1
Coordinates 1257899..1258213 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID D3GWQ0
Locus tag I6K21_RS05710 Protein ID WP_000560253.1
Coordinates 1257486..1257902 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K21_RS05700 (1252901) 1252901..1255252 + 2352 WP_000695439.1 alpha-glucosidase -
I6K21_RS05705 (1255423) 1255423..1257441 + 2019 WP_000121438.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
I6K21_RS05710 (1257486) 1257486..1257902 - 417 WP_000560253.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
I6K21_RS05715 (1257899) 1257899..1258213 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
I6K21_RS05720 (1258498) 1258498..1259634 - 1137 WP_000018677.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
I6K21_RS05725 (1259719) 1259719..1260222 + 504 WP_001309773.1 M48 family metallopeptidase -
I6K21_RS05730 (1260299) 1260299..1260982 + 684 WP_001183054.1 vancomycin high temperature exclusion protein -
I6K21_RS05735 (1261061) 1261061..1262047 + 987 WP_001377074.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T192465 WP_000550189.1 NZ_CP069980:c1258213-1257899 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T192465 NZ_CP092417:c1203574-1203152 [Endozoicomonas sp. SCSIO W0465]
ATGAATGACATTATTCTTAACAAATACTCAATCATTCAGCGTTGTATTGCCCGTATTAATGAAGAATACAAAGGTCATGA
GTCGGAACTGGCCACAAATTACACTCAGCAGGATTCTATAATTCTGAATATTCAGAGAGCGTGCCAGGCCGCCCAGGATA
TGGCGAACTACGTTATCAAACTGAAAAAATTGGGAATCCCCCAGAATGCCAGAGAATCTTTCCAGCTACTAAACGATCAT
GGACTGGTATCAGCCCCACTCACAGTCAGTATGATAAAAATGGTGGGGTTCAGGAATATAGCTGTCCATGAGTACCAAAA
ATTGAACATAGCTATTCTCAAATCTGTTATAGAAAACCATGTTGATGAAATTAACGGGTTCGCAGAGGAAGTTATGTGCA
GTTTTAATAATGATGGACAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14965.39 Da        Isoelectric Point: 4.4547

>AT192465 WP_000560253.1 NZ_CP069980:c1257902-1257486 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT192465 NZ_CP092417:c1203971-1203567 [Endozoicomonas sp. SCSIO W0465]
ATGGAACCACTGTGCAAGGCAGTCATCGTATCATTTCTCAGGCAAGCTTATGGTGACTTGTTACAAGGTATACTGCTCTT
TGGGAGCCATGCCAACGGCACAGCCAATAAAGAAAGTGACATAGACTTGGGTATTTTGATTGATGGTATGGCTGACCCGG
TTGAACTATGGGAAAACGCCCAGACACTAGCCAGCCAGGTGAATACCAATATTGACCTGATAGATTTACGCGCAGCAACC
ACTGTACTACAGCATGAAGTGATCAACACAGGACTCTGGCTGTGGCAGCAAGATACCCTGACTTGTGACTTATTCGAACT
CCAGGTTATGTCGTTGTACCAGCAACTACAATACGATAGACGGGAAATCCTGGACGACTTAAAAAGAAGGCTGCGTAATG
AATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure
AlphaFold DB A0A829IXD3

References