Detailed information of TA system TA192451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3660457..3661059
• Replicon: chromosome
• Accession: NZ_CP069978
• Organism: Escherichia coli strain FDAARGOS_1299

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6K29_RS17600
• Protein ID: WP_000897302.1
• Coordinates: 3660748..3661059 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K29_RS17595
• Protein ID: WP_000356397.1
• Coordinates: 3660457..3660747 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K29_RS17570 (3656530)	3656530..3657432	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
I6K29_RS17575 (3657429)	3657429..3658064	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K29_RS17580 (3658061)	3658061..3658990	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6K29_RS17585 (3659206)	3659206..3659424	-	219	WP_001314326.1	CopG family transcriptional regulator	-
I6K29_RS17590 (3659820)	3659820..3660098	-	279	WP_001296612.1	hypothetical protein	-
I6K29_RS17595 (3660457)	3660457..3660747	-	291	WP_000356397.1	NadS family protein	Antitoxin
I6K29_RS17600 (3660748)	3660748..3661059	-	312	WP_000897302.1	hypothetical protein	Toxin
I6K29_RS17605 (3661288)	3661288..3662196	+	909	WP_001331553.1	alpha/beta hydrolase	-
I6K29_RS17610 (3662260)	3662260..3663201	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K29_RS17615 (3663246)	3663246..3663683	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
I6K29_RS17620 (3663680)	3663680..3664552	-	873	WP_000920762.1	virulence factor BrkB family protein	-
I6K29_RS17625 (3664546)	3664546..3665145	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T192451 WP_000897302.1 NZ_CP069978:c3661059-3660748 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T192451 NZ_CP092407:128404-128715 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT192451 WP_000356397.1 NZ_CP069978:c3660747-3660457 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT192451 NZ_CP092407:128712-129131 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: S1P416