Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1335332..1335894 Replicon chromosome
Accession NZ_CP069978
Organism Escherichia coli strain FDAARGOS_1299

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I6K29_RS06380 Protein ID WP_000605675.1
Coordinates 1335332..1335610 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6K29_RS06385 Protein ID WP_000781370.1
Coordinates 1335610..1335894 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K29_RS06355 (1330919) 1330919..1331350 - 432 WP_000152310.1 peroxiredoxin OsmC -
I6K29_RS06360 (1331695) 1331695..1331910 + 216 WP_000495766.1 biofilm-dependent modulation protein -
I6K29_RS06365 (1332012) 1332012..1332149 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6K29_RS06370 (1332305) 1332305..1334002 + 1698 WP_000433462.1 malate dehydrogenase -
I6K29_RS06375 (1334136) 1334136..1335146 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
I6K29_RS06380 (1335332) 1335332..1335610 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K29_RS06385 (1335610) 1335610..1335894 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
I6K29_RS06390 (1336123) 1336123..1336776 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
I6K29_RS06395 (1336769) 1336769..1337653 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
I6K29_RS06400 (1337666) 1337666..1340713 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T192442 WP_000605675.1 NZ_CP069978:1335332-1335610 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T192442 NZ_CP092400:11346-11762 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT192442 WP_000781370.1 NZ_CP069978:1335610-1335894 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT192442 NZ_CP092400:11119-11349 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAAGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References