Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 716695..717197 Replicon chromosome
Accession NZ_CP069978
Organism Escherichia coli strain FDAARGOS_1299

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag I6K29_RS03420 Protein ID WP_000767822.1
Coordinates 716943..717197 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I6K29_RS03415 Protein ID WP_001259255.1
Coordinates 716695..716946 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K29_RS03390 (711873) 711873..712940 - 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
I6K29_RS03395 (712940) 712940..714010 - 1071 WP_000108995.1 histidinol-phosphate transaminase -
I6K29_RS03400 (714007) 714007..715311 - 1305 WP_001297912.1 histidinol dehydrogenase -
I6K29_RS03405 (715317) 715317..716216 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
I6K29_RS03410 (716362) 716362..716412 - 51 WP_001364200.1 his operon leader peptide -
I6K29_RS03415 (716695) 716695..716946 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I6K29_RS03420 (716943) 716943..717197 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I6K29_RS03425 (717280) 717280..718104 + 825 WP_000754733.1 SDR family oxidoreductase -
I6K29_RS03430 (718150) 718150..719079 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
I6K29_RS03435 (719294) 719294..719356 + 63 WP_010723108.1 membrane protein YoeI -
I6K29_RS03440 (719346) 719346..720704 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T192431 WP_000767822.1 NZ_CP069978:716943-717197 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T192431 NZ_CP092383:471370-471720 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT192431 WP_001259255.1 NZ_CP069978:716695-716946 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT192431 NZ_CP092383:471083-471364 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References