Detailed information of TA system TA192424

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 61808..62047
• Replicon: plasmid unnamed4
• Accession: NZ_CP069974
• Organism: Escherichia coli strain FDAARGOS_1280

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: I6K10_RS26145
• Protein ID: WP_023144756.1
• Coordinates: 61808..61942 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	61987..62047 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K10_RS26115 (57509)	57509..58366	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
I6K10_RS26120 (58359)	58359..58433	-	75	WP_031943482.1	RepA leader peptide Tap	-
I6K10_RS26130 (58795)	58795..60336	+	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
I6K10_RS26135 (60351)	60351..61097	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
I6K10_RS26140 (61257)	61257..61511	-	255	WP_000083850.1	replication regulatory protein RepA	-
I6K10_RS26145 (61808)	61808..61942	-	135	WP_023144756.1	Hok/Gef family protein	Toxin
- (61987)	61987..62047	+	61	NuclAT_2	-	Antitoxin
- (61987)	61987..62047	+	61	NuclAT_2	-	Antitoxin
- (61987)	61987..62047	+	61	NuclAT_2	-	Antitoxin
- (61987)	61987..62047	+	61	NuclAT_2	-	Antitoxin
I6K10_RS26150 (62014)	62014..62300	-	287	Protein_85	DUF2726 domain-containing protein	-
I6K10_RS26155 (62813)	62813..63025	-	213	WP_013023861.1	hypothetical protein	-
I6K10_RS26160 (63156)	63156..63716	-	561	WP_000139341.1	fertility inhibition protein FinO	-
I6K10_RS26165 (63771)	63771..64517	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
I6K10_RS26170 (64537)	64537..66993	-	2457	Protein_89	conjugative transfer relaxase/helicase TraI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(B) / dfrA17 / aadA5 / qacE / sul1 / blaTEM-1B / blaCTX-M-15 / qnrS1 / erm(B) / mph(A) / aac(3)-IId	-	1..136429	136429

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T192424 WP_023144756.1 NZ_CP069974:c61942-61808 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T192424 NZ_CP092381:c4099122-4098199 [Xanthomonas oryzae]
ATGAACGAACCTGACAAGAGCAGGCGCACTGTTGCCGCGTCGGTTCGCGCCCGGCTGCTCAACAAGGCCCGCGCCGAAAG
ACTCGACTTCAACCTGCTGCTGACCCGCTACGCGCTGGAGCGCGTCCTTTACCGCCTGAGCGTTTCGGAGCAGCAGGGTC
AATTCTTGCTCAAAGGTGCGCTGCTGTTCGACATCTGGTTCGACATACCGCATCGCCCGACGCACGATGCCGACCTGCTG
GGCTTCGGCTCCACCGAGATCCCACATCTGGAAGACCTGTTCCGCAGAATCAGTCAAATCGCCAGCGATGACGGCATCGT
GTTTCAGGCCGACAGCGTGAAGGCCGCCGAAATCCGCAAGGAGGCCAACTACGCGGGCGTCCGTGTGACGATGATCGGCC
TGCTCGATGGCGCGCGCTGCACGGTGCAGATCGACATCGGTTTCGGCGACGCCGTGACGCCCGGCCCGGACAACGTGCAG
TACCCAGTCATCCTCGACGGCATGCCGCAGCCGCAACTGCGGGTCTATCCCCGCTACACCGTGGTCGCCGAAAAGCTGGA
AGCGATGGTCAAGCTCGGCATCCTCAACAGCCGGATGAAGGACTACTTCGACCTCTGGGTTCTCGCCGGACATTCCGACT
TCGACGGTGCGGTGCTGGCCACGGCCATTCGCGCCACGTTCGAGTGCAGGGGTACGGCCATCCCACCCGGCGCGCCGCTG
GGCCTCACCGATGAGTTCGGCCTGGACGAGCAGAAGACAAAGCAGTGGCAGGCGTTCCTGCGCAAGAACACCTTGGAGCC
GATGCCGCTGACCACGGTGATCGAAGCGCTGCGCGAATTCGTGCTGCCCGTGCTGGCCGCGCTCGCGGCAGGCGAAGGTT
TCGACCGCCAGTGGCGCGCGGGCGCGGGATGGGATGCGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT192424 NZ_CP069974:61987-62047 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin