Detailed information of TA system TA192400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2337391..2338085
• Replicon: chromosome
• Accession: NZ_CP069973
• Organism: Escherichia coli strain FDAARGOS_1280

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: I6K10_RS12060
• Protein ID: WP_001521903.1
• Coordinates: 2337687..2338085 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6K10_RS12055
• Protein ID: WP_000554758.1
• Coordinates: 2337391..2337684 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K10_RS12030 (2332506)	2332506..2333255	+	750	WP_016233951.1	C40 family peptidase	-
I6K10_RS12035 (2333312)	2333312..2333665	-	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
I6K10_RS12040 (2333744)	2333744..2335456	-	1713	Protein_2174	flagellar biosynthesis protein FlhA	-
I6K10_RS12045 (2335428)	2335428..2336213	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
I6K10_RS12050 (2336284)	2336284..2337339	+	1056	WP_001226177.1	DNA polymerase IV	-
I6K10_RS12055 (2337391)	2337391..2337684	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6K10_RS12060 (2337687)	2337687..2338085	+	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6K10_RS12065 (2338095)	2338095..2338547	+	453	WP_023144376.1	GNAT family N-acetyltransferase	-
I6K10_RS12070 (2338792)	2338792..2338998	+	207	Protein_2180	RtcB family protein	-
I6K10_RS12075 (2338994)	2338994..2339346	+	353	Protein_2181	peptide chain release factor H	-
I6K10_RS12080 (2339403)	2339403..2340860	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
I6K10_RS12085 (2341121)	2341121..2341579	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (2342175)	2342175..2342255	+	81	NuclAT_11	-	-
- (2342175)	2342175..2342255	+	81	NuclAT_11	-	-
- (2342175)	2342175..2342255	+	81	NuclAT_11	-	-
- (2342175)	2342175..2342255	+	81	NuclAT_11	-	-
I6K10_RS12090 (2341671)	2341671..2342915	+	1245	WP_000189541.1	esterase FrsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T192400 WP_001521903.1 NZ_CP069973:2337687-2338085 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T192400 NZ_CP092373:1462867-1463145 [Pantoea sp. Z09]
ATGGCTAAGCAAAGGGAAATTGCCTACTCAAACCAGTTTTACAAAGACGTCAAAAAGATGCAGAAGCGACGCAAAAAGAT
GGACAAGCTTAAAACGCTTATGTCGCTGCTTATGAACGATAGATTGCCTCTTCCTGCGGCTTATAAAGATCATCCGCTGC
AGGGTAACTACAGGGGTTACAGAGATGCGCATATTGAACCCGACTGGCTACTGATCTACAAACTAACGGATGAGCTAATA
AGGTTCGAAAGAACCGGTACGCACGCAGACCTGTTTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT192400 WP_000554758.1 NZ_CP069973:2337391-2337684 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT192400 NZ_CP092373:1462578-1462838 [Pantoea sp. Z09]
ATGCCCACCAATGCATTCGTACGTGCTCGTATAGACGAGACATTGAAAAATGAAGCCGCCGCCGTGCTGGCTGAAATGGG
ATTAACCGTTTCTGACCTGGTACGCATCACCCTGACTAAAGTTGCCAAAGAAAAGGCGCTGCCTTTTGATCTGCGCATCC
CCAACGACATCACAGCCAGCAGCATCGAAGCGAGCGAGCAAGGCAAAGGCGTAAAAAAAGCGAAGGATGAAAAAGATCTG
TTTGACCAGTTAGGTATTTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3