Detailed information of TA system TA192394

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1375630..1376232
• Replicon: chromosome
• Accession: NZ_CP069973
• Organism: Escherichia coli strain FDAARGOS_1280

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: I6K10_RS07515
• Protein ID: WP_000897305.1
• Coordinates: 1375630..1375941 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K10_RS07520
• Protein ID: WP_000356395.1
• Coordinates: 1375942..1376232 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K10_RS07495 (1370671)	1370671..1371108	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
I6K10_RS07500 (1371153)	1371153..1372094	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K10_RS26965 (1372510)	1372510..1373397	+	888	Protein_1280	hypothetical protein	-
I6K10_RS26970 (1373410)	1373410..1374324	+	915	WP_077634137.1	transposase	-
I6K10_RS07510 (1374493)	1374493..1375401	-	909	WP_001306651.1	alpha/beta hydrolase	-
I6K10_RS07515 (1375630)	1375630..1375941	+	312	WP_000897305.1	hypothetical protein	Toxin
I6K10_RS07520 (1375942)	1375942..1376232	+	291	WP_000356395.1	NadS family protein	Antitoxin
I6K10_RS07525 (1376317)	1376317..1376529	+	213	WP_000197769.1	hypothetical protein	-
I6K10_RS07530 (1376591)	1376591..1376869	+	279	WP_001306650.1	hypothetical protein	-
I6K10_RS07535 (1377288)	1377288..1377506	+	219	WP_001295676.1	CopG family transcriptional regulator	-
I6K10_RS07540 (1377725)	1377725..1377967	+	243	WP_021523315.1	hypothetical protein	-
I6K10_RS07545 (1378149)	1378149..1379078	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
I6K10_RS07550 (1379075)	1379075..1379710	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K10_RS07555 (1379707)	1379707..1380609	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T192394 WP_000897305.1 NZ_CP069973:1375630-1375941 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T192394 NZ_CP092369:2629024-2629203 [Bacillus subtilis]
ATGTCGACCTATGAATCTCTAATGGTCATGATCGGCTTTGCCAATTTAATAGGCGGGATTATGACATGGGTAATATCTCT
TTTAACATTATTATACATGCTTAGAAAAAAAGACACTCATCCTATTTACATTACTGTAAAGGAAAAGTGTCTACACGAGG
ACCCTCCTATTAAAGGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT192394 WP_000356395.1 NZ_CP069973:1375942-1376232 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT192394 NZ_CP092369:c2629349-2629127 [Bacillus subtilis]
CAAGCAAAAGTATTGCAACTACTGGCGTAAGCTTATAGTTGGTACCACATTACCACATTACCACTTGTTAATGTGTGTTA
CTTTCATGAGAAATGCTAAAATATAAAAGCCAGAGTGTGGCAGCACTCTAGCTTTTAAAAAAGAAACTACCCTTTAATAG
GAGGGTCCTCGTGTAGACACTTTTCCTTTACAGTAATGTAAATAGGATGAGTGTCTTTTTTTC

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7