Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 547561..548360 Replicon chromosome
Accession NZ_CP069973
Organism Escherichia coli strain FDAARGOS_1280

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag I6K10_RS03610 Protein ID WP_000347272.1
Coordinates 547896..548360 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag I6K10_RS03605 Protein ID WP_001307405.1
Coordinates 547561..547896 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K10_RS03590 (543346) 543346..544116 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
I6K10_RS03595 (544132) 544132..545466 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
I6K10_RS03600 (545841) 545841..547412 + 1572 WP_001273738.1 galactarate dehydratase -
I6K10_RS03605 (547561) 547561..547896 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
I6K10_RS03610 (547896) 547896..548360 + 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
I6K10_RS03615 (548415) 548415..549224 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
I6K10_RS03620 (549473) 549473..550753 + 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
I6K10_RS03625 (550776) 550776..551249 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
I6K10_RS03630 (551260) 551260..552039 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
I6K10_RS03635 (552029) 552029..552907 + 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
I6K10_RS03640 (552925) 552925..553359 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 538036..548360 10324


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T192393 WP_000347272.1 NZ_CP069973:547896-548360 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T192393 NZ_CP092369:2232321-2232437 [Bacillus subtilis]
ATGACTGTTTACGAATCATTAATGATAATGATCAATTTTGGCGGATTGATATTAAATACCGTCTTGTTGATCTTCAATAT
AATGATGATTGTAACGTCAAGCCAAAAGAAAAAATAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT192393 WP_001307405.1 NZ_CP069973:547561-547896 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT192393 NZ_CP092369:c2232485-2232432 [Bacillus subtilis]
AAATCTGCGTAGGCCTAACCCTTCAGGTGTCCAAACTCAAGGGAAGGTCTATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References