Detailed information of TA system TA192388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 67813..68540
• Replicon: chromosome
• Accession: NZ_CP069973
• Organism: Escherichia coli strain FDAARGOS_1280

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: I6K10_RS01330
• Protein ID: WP_001521139.1
• Coordinates: 68229..68540 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K10_RS01325
• Protein ID: WP_000126294.1
• Coordinates: 67813..68232 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K10_RS01305 (62898)	62898..63425	-	528	WP_001078777.1	electron transport protein HydN	-
I6K10_RS01310 (63574)	63574..64584	-	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
I6K10_RS01315 (64844)	64844..66301	+	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
I6K10_RS01320 (66310)	66310..67734	+	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
I6K10_RS01325 (67813)	67813..68232	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
I6K10_RS01330 (68229)	68229..68540	-	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6K10_RS01335 (68707)	68707..69177	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
I6K10_RS01340 (69170)	69170..69580	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
I6K10_RS01345 (69577)	69577..70344	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
I6K10_RS01350 (70344)	70344..70886	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
I6K10_RS01355 (70896)	70896..72605	-	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T192388 WP_001521139.1 NZ_CP069973:c68540-68229 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T192388 NZ_CP092369:510154-510504 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT192388 WP_000126294.1 NZ_CP069973:c68232-67813 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT192388 NZ_CP092369:509868-510149 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A829L2T9