Detailed information of TA system TA192384

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26028..26292
• Replicon: plasmid unnamed3
• Accession: NZ_CP069972
• Organism: Escherichia coli strain FDAARGOS_1280

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: I6K10_RS00660
• Protein ID: WP_001331364.1
• Coordinates: 26028..26180 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	26235..26292 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K10_RS00635 (21305)	21305..23473	+	2169	WP_000698357.1	DotA/TraY family protein	-
I6K10_RS00640 (23547)	23547..24197	+	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
I6K10_RS00645 (24269)	24269..24478	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
I6K10_RS00650 (24870)	24870..25046	+	177	WP_001054898.1	hypothetical protein	-
I6K10_RS27230 (25111)	25111..25206	-	96	WP_001303310.1	DinQ-like type I toxin DqlB	-
I6K10_RS00655 (25705)	25705..25956	+	252	WP_001291964.1	hypothetical protein	-
I6K10_RS00660 (26028)	26028..26180	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
- (26235)	26235..26292	+	58	NuclAT_0	-	Antitoxin
- (26235)	26235..26292	+	58	NuclAT_0	-	Antitoxin
- (26235)	26235..26292	+	58	NuclAT_0	-	Antitoxin
- (26235)	26235..26292	+	58	NuclAT_0	-	Antitoxin
- (26533)	26533..26588	+	56	NuclAT_1	-	-
- (26533)	26533..26588	+	56	NuclAT_1	-	-
- (26533)	26533..26588	+	56	NuclAT_1	-	-
- (26533)	26533..26588	+	56	NuclAT_1	-	-
I6K10_RS00665 (26782)	26782..27735	+	954	WP_021513958.1	hypothetical protein	-
I6K10_RS00670 (27953)	27953..29161	+	1209	WP_048257178.1	IncI1-type conjugal transfer protein TrbA	-
I6K10_RS00675 (29180)	29180..30250	+	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..83090	83090

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T192384 WP_001331364.1 NZ_CP069972:c26180-26028 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T192384 NZ_CP092364:307781-308224 [Mycolicibacterium goodii]
ATGGCCAAACTTTCCGTCTCCGTCGAAGTCCCATTGCCGCCGGAGCAGGCATGGGAGTACGCCTCCGACCTGTCCCGGTA
CCACGAATGGCTCAGCATTCACCGGGCGTGGCGCTCGAAGCTGCCCGAAACCCTGGAGAAGGGCACGGTGATCGAGTCGA
TCGTCGAGGTCAAGGGCATGCTCAACCGGATCAGGTGGACGCTGGTCCACTACAAGCCCCCGCAGGCGCTGACGCTCAAT
GGCGACGGCCGCGGCGGGGTCAAGGTCAAGCTGATCGGCAAGATCAAGCCGACCGACAACGGCTCGATCGTGGGGTTTGA
CTTGCATCTGGGTGGTCCGGCGCTGTTCGGGCCCATCGGCATGGTGGTTGCCGCGGCGCTCAAGAGCGACATCCAGGAGT
CGCTCAACCGCTTCAAGGCGCTCTACGCCCCGTCCGCGACGTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT192384 NZ_CP069972:26235-26292 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin