Detailed information of TA system TA192383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 10759..11405
• Replicon: plasmid unnamed2
• Accession: NZ_CP069971
• Organism: Escherichia coli strain FDAARGOS_1280

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I6K10_RS00270
• Protein ID: WP_000269912.1
• Coordinates: 11058..11405 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K10_RS00265
• Protein ID: WP_001673379.1
• Coordinates: 10759..11058 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K10_RS00235 (I6K10_00235)	6782..6973	+	192	WP_000183352.1	hypothetical protein	-
I6K10_RS00240 (I6K10_00240)	6970..8151	+	1182	WP_204638442.1	ORF6N domain-containing protein	-
I6K10_RS00245 (I6K10_00245)	8186..8821	-	636	WP_204638443.1	hypothetical protein	-
I6K10_RS00250 (I6K10_00250)	9457..9729	-	273	WP_000148349.1	helix-turn-helix domain-containing protein	-
I6K10_RS00255 (I6K10_00255)	9793..10173	-	381	WP_000061763.1	hypothetical protein	-
I6K10_RS00260 (I6K10_00260)	10441..10725	+	285	WP_021542820.1	hypothetical protein	-
I6K10_RS00265 (I6K10_00265)	10759..11058	-	300	WP_001673379.1	XRE family transcriptional regulator	Antitoxin
I6K10_RS00270 (I6K10_00270)	11058..11405	-	348	WP_000269912.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K10_RS00275 (I6K10_00275)	11650..11913	-	264	WP_000424604.1	hypothetical protein	-
I6K10_RS00280 (I6K10_00280)	11937..12224	-	288	WP_000356589.1	hypothetical protein	-
I6K10_RS00285 (I6K10_00285)	12868..13848	-	981	WP_241336524.1	plasmid replication initiator RepA	-
I6K10_RS00290 (I6K10_00290)	14056..14583	-	528	WP_204638444.1	tail fiber assembly protein	-
I6K10_RS00295 (I6K10_00295)	14585..15625	-	1041	WP_251296402.1	phage tail protein	-
I6K10_RS00300 (I6K10_00300)	15635..15913	-	279	WP_032152803.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48644	48644

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13545.51 Da
• Isoelectric Point: 8.8337

>T192383 WP_000269912.1 NZ_CP069971:c11405-11058 [Escherichia coli]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T192383 NZ_CP092363:c107113-106835 [Mycolicibacterium crocinum]
GTGAACGCCGAAGACGGCTACACGACACGTTTCACCGCAACGGCGCGGCGCGATCTCGACAAGCTGCCGCCGCGCATCCT
GGCGGCGGTCATCGAGTTCGCCTTCGGCGACCTCACGCGAGAACCCCGCAGAGTCGGCAAACCCCTCGGTGGCAAGCTGG
CCGGTCAGTTCAGCGCCAGGCGCGGCCCCTACCGCCTCCTCTACCGCATCGACGACCCCGCGGCGACCATCTGGGTCCAC
CGCGTCGACCACCGCTCGGACGTATACCGGCGTCCCTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11069.90 Da
• Isoelectric Point: 5.8899

>AT192383 WP_001673379.1 NZ_CP069971:c11058-10759 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRVAFHV

Nucleotide

• Length: 300 bp

>AT192383 NZ_CP092363:c107396-107115 [Mycolicibacterium crocinum]
ATGAGGATATTGCCGATATCGCAGCTAAAAAGCAAGATCAACGAGTATGTCGATGCGGTCTCTGATACGCGTGACCAGAT
CACCATCACCAAAAACGGTGCGCCGGCAGCCGTGCTGATCGGCGTTGATGAATGGGAGTCGATACAAGAGACGCTGCACT
GGCTGTCGCAGCCCGGCATCGTCGAATCGGTCTCCGAGTCCGAGTCCGACATCGCTGCCGGTCGCACCTACGGTGAGGAC
GAGATCCGCGCCGCTTACCAGGTTCCACGGCGGCCGCGCTGA