Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2607932..2608529 Replicon chromosome
Accession NZ_CP069968
Organism Klebsiella pneumoniae strain FDAARGOS_1308

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag I6K38_RS13015 Protein ID WP_004142563.1
Coordinates 2608212..2608529 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag I6K38_RS13010 Protein ID WP_004142561.1
Coordinates 2607932..2608219 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K38_RS12980 2604012..2604260 + 249 WP_002893037.1 DUF1158 domain-containing protein -
I6K38_RS12985 2604278..2604619 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
I6K38_RS12990 2604650..2605765 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
I6K38_RS12995 2605945..2606526 + 582 WP_204752821.1 TetR family transcriptional regulator -
I6K38_RS13000 2606526..2606894 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
I6K38_RS13005 2607014..2607667 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
I6K38_RS13010 2607932..2608219 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
I6K38_RS13015 2608212..2608529 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K38_RS13020 2608714..2609757 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
I6K38_RS13025 2610424..2611290 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
I6K38_RS13030 2611399..2612826 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T192371 WP_004142563.1 NZ_CP069968:c2608529-2608212 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T192371 NZ_CP092359:974948-975277 [Wielerella bovis]
ATGGAGCGTGGCGATATTTTTCTGGTCAATTTAGACCCCACAACAGGGCATGAACAACAAGGCACACGCCCAGTTCTCAT
TATTTCCCCGAAAGCATTCAATGCTTTAACCCAAGTGCCGATTGTTTTGCCGATTACGACCAAAAGCCAATTTGCGCGAA
CGCAAGGTTTTGCCGTGCATTTGGACGAGCGTACCAAAACGCAAGGCATTGTCCGCACAGACCAGTCACGCGCTTTGGAT
ATTCAGGCGCGAAATGGCAGGAAATTGGAAACCTTACCGATTGATTTAATGAATGAAGTGTTGGCAAAAATGGCGACTTT
TTTAAGCTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT192371 WP_004142561.1 NZ_CP069968:c2608219-2607932 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT192371 NZ_CP092359:974696-974941 [Wielerella bovis]
ATGTACACCGTTTCCCTACGCAAAGTTGGCGGTTCAACCATGCTTGCCATTCCACCAGCTTTATTGCAAAGCCTTGATTT
GGGAAAACAAGTTCATTTGCATACCGAAAATGGACGTTTGATTATTGAGCCAGCGCGTCCCCAATACACTTTGGCGGAAT
TGCTCGCACAATGCGACCCCAACGCGCCACGCGATGTGGAAAGTCAAACGTGGTTGGACGCGCCTGCCGTTGGGCAAGAA
TGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References