Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3481749..3482346 Replicon chromosome
Accession NZ_CP069962
Organism Klebsiella pneumoniae strain FDAARGOS_1307

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag I6K37_RS17045 Protein ID WP_004142563.1
Coordinates 3482029..3482346 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag I6K37_RS17040 Protein ID WP_004142561.1
Coordinates 3481749..3482036 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K37_RS17010 3477829..3478077 + 249 WP_002893037.1 DUF1158 domain-containing protein -
I6K37_RS17015 3478095..3478436 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
I6K37_RS17020 3478467..3479582 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
I6K37_RS17025 3479762..3480343 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
I6K37_RS17030 3480343..3480711 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
I6K37_RS17035 3480831..3481484 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
I6K37_RS17040 3481749..3482036 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
I6K37_RS17045 3482029..3482346 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K37_RS17050 3482531..3483574 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
I6K37_RS17055 3484241..3485107 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
I6K37_RS17060 3485216..3486643 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T192350 WP_004142563.1 NZ_CP069962:c3482346-3482029 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T192350 NZ_CP092336:c5274040-5273657 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT192350 WP_004142561.1 NZ_CP069962:c3482036-3481749 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT192350 NZ_CP092336:c5274282-5274040 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References