Detailed information of TA system TA192318

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 119489..119915
• Replicon: plasmid unnamed2
• Accession: NZ_CP069959
• Organism: Escherichia coli strain FDAARGOS_1279

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: I6K09_RS25620
• Protein ID: WP_001372321.1
• Coordinates: 119489..119614 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	119691..119915 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K09_RS25595 (114863)	114863..115552	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
I6K09_RS25600 (115739)	115739..116122	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
I6K09_RS25605 (116455)	116455..117045	+	591	WP_192849409.1	transglycosylase SLT domain-containing protein	-
I6K09_RS25610 (117342)	117342..118163	-	822	WP_001234426.1	DUF932 domain-containing protein	-
I6K09_RS25615 (118281)	118281..118568	-	288	WP_000107535.1	hypothetical protein	-
I6K09_RS26065 (118593)	118593..118799	-	207	WP_000547968.1	hypothetical protein	-
I6K09_RS26070 (118869)	118869..119041	+	173	Protein_147	hypothetical protein	-
I6K09_RS26075 (119039)	119039..119269	-	231	WP_071886920.1	hypothetical protein	-
I6K09_RS25620 (119489)	119489..119614	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6K09_RS26080 (119556)	119556..119705	-	150	Protein_150	plasmid maintenance protein Mok	-
- (119691)	119691..119915	-	225	NuclAT_0	-	Antitoxin
- (119691)	119691..119915	-	225	NuclAT_0	-	Antitoxin
- (119691)	119691..119915	-	225	NuclAT_0	-	Antitoxin
- (119691)	119691..119915	-	225	NuclAT_0	-	Antitoxin
I6K09_RS25625 (119727)	119727..119915	+	189	WP_001299721.1	hypothetical protein	-
I6K09_RS25630 (119884)	119884..120646	-	763	Protein_152	plasmid SOS inhibition protein A	-
I6K09_RS25635 (120643)	120643..121077	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
I6K09_RS25640 (121132)	121132..123090	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
I6K09_RS25645 (123149)	123149..123382	-	234	WP_000006004.1	DUF905 domain-containing protein	-
I6K09_RS25650 (123747)	123747..124568	+	822	WP_001272932.1	abortive infection system antitoxin AbiGi family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B	-	1..135867	135867

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T192318 WP_001372321.1 NZ_CP069959:c119614-119489 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T192318 NZ_CP092288:c4551353-4551075 [Marinobacter sp. M2C]
ATGATTCGAAGCTTCAAACACAAGGGCTTGGCCAAATTCTTCAAGTCCGGCAGCATCGCAGGGATTCAGGCCGCTCACGC
CAAGAGGCTTCGGCTTATTCTCGGTAGATTAAATGCAGCCTCAGATGCCAAAGATATGGACCTGCCAGGCCTTCGGTTGC
ACGAACTCTCCGGTAATCGCGCCGGAATCTGGTCAGTGATGGTCAGTGGCAACTGGCGAGTGACGTTCCGATTTGAGGAC
GGAGATGCCGAGATCGTAAATTATGAAGATTACCATTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT192318 NZ_CP069959:c119915-119691 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin