Detailed information of TA system TA192314

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 79413..79667
• Replicon: plasmid unnamed2
• Accession: NZ_CP069959
• Organism: Escherichia coli strain FDAARGOS_1279

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: I6K09_RS25390
• Protein ID: WP_001312851.1
• Coordinates: 79413..79562 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	79606..79667 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K09_RS25360 (74660)	74660..75571	-	912	WP_000440183.1	carbamate kinase	-
I6K09_RS25365 (75582)	75582..76802	-	1221	WP_000410951.1	arginine deiminase	-
I6K09_RS25370 (77509)	77509..78123	+	615	Protein_96	VENN motif pre-toxin domain-containing protein	-
I6K09_RS25375 (78123)	78123..78569	-	447	Protein_97	plasmid replication initiator RepA	-
I6K09_RS25380 (78562)	78562..78636	-	75	WP_032336874.1	RepA leader peptide Tap	-
I6K09_RS25385 (78872)	78872..79129	-	258	WP_000083833.1	replication regulatory protein RepA	-
I6K09_RS25390 (79413)	79413..79562	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (79606)	79606..79667	+	62	NuclAT_1	-	Antitoxin
- (79606)	79606..79667	+	62	NuclAT_1	-	Antitoxin
- (79606)	79606..79667	+	62	NuclAT_1	-	Antitoxin
- (79606)	79606..79667	+	62	NuclAT_1	-	Antitoxin
I6K09_RS25395 (79806)	79806..79988	-	183	WP_000968309.1	hypothetical protein	-
I6K09_RS25400 (80089)	80089..80705	+	617	Protein_102	IS1-like element IS1A family transposase	-
I6K09_RS25405 (80743)	80743..82314	-	1572	WP_100190670.1	IS66 family transposase	-
I6K09_RS25410 (82334)	82334..82681	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
I6K09_RS25415 (82681)	82681..83358	-	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
I6K09_RS25420 (83413)	83413..83502	+	90	Protein_106	IS1 family transposase	-
I6K09_RS25425 (83803)	83803..84015	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B	-	1..135867	135867

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T192314 WP_001312851.1 NZ_CP069959:c79562-79413 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T192314 NZ_CP092288:c4521685-4521512 [Marinobacter sp. M2C]
ATGGACGAGTTTGAGTTCGACGAAACTAAAAGTCAGACCAATCTGGATAAGCACGGCATTGATTTCGTGGCCGCCCGAGT
TCTGTGGAAAGATCCGTATCTATTGGAAATCCGCGCAAAATCAGAGGGCGAGCCAAGATTCGTGTTGATAGGCAAGATTG
GGGAAAGACACTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT192314 NZ_CP069959:79606-79667 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin