Detailed information of TA system TA192308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4483144..4483871
• Replicon: chromosome
• Accession: NZ_CP069957
• Organism: Escherichia coli strain FDAARGOS_1279

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: I6K09_RS21465
• Protein ID: WP_001521139.1
• Coordinates: 4483144..4483455 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K09_RS21470
• Protein ID: WP_000126294.1
• Coordinates: 4483452..4483871 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K09_RS21440 (4479079)	4479079..4480788	+	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-
I6K09_RS21445 (4480798)	4480798..4481340	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
I6K09_RS21450 (4481340)	4481340..4482107	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
I6K09_RS21455 (4482104)	4482104..4482514	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
I6K09_RS21460 (4482507)	4482507..4482977	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
I6K09_RS21465 (4483144)	4483144..4483455	+	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6K09_RS21470 (4483452)	4483452..4483871	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
I6K09_RS21475 (4483950)	4483950..4485374	-	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
I6K09_RS21480 (4485383)	4485383..4486840	-	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
I6K09_RS21485 (4487100)	4487100..4488110	+	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
I6K09_RS21490 (4488259)	4488259..4488786	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T192308 WP_001521139.1 NZ_CP069957:4483144-4483455 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T192308 NZ_CP092288:c1914402-1914136 [Marinobacter sp. M2C]
ATGATTAGATCATTCCGTCACAAAGGTCTGAAACGGTTCTACTCGTCTGGCATACAGTCTGATCACGCCAAGAAGCTGCG
CATGCAGCTCGCTGCTCTGAATACCGCCATTTCGGTAGAAGACATAGACATCCCTGGTTTTCGGCTTCACTCATTGAAGG
GAACAGACAAAGGGCGGTGGTCGATTTGGGTTAACGGAAATTGGCGCATGACGTTCGAATTTCAGGACAGCAACGCCTAC
ATTCTTGATTATGAGGATTACCACTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT192308 WP_000126294.1 NZ_CP069957:4483452-4483871 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT192308 NZ_CP092288:c1914136-1913843 [Marinobacter sp. M2C]
ATGACTATGCATAACCCGCCACATCCTGGTGAGTTCATTCGGGAGGTGTATCTGGAGCCTTTCGGCATCAGTTCCCGTCA
GCTTGCTTCCAGTTTGGGCGTTTCCCCTTCTACGTTGTCTCGCTTGCTCAAAGGGGATAGTGGTATCAGCCCGGAGATGT
CTTTGCGGCTATCTAAGGTTCTCGGGCGTACTCCTGAGAGCTGGTTGGCTATGCAAGATATGTATGATCTATGGGTAGCC
CGTAGCACGGTCAATCTGGATGGTATTCATCCCTTGGACTTCGAAGCAGCTTAA

Structures

Toxin

• AlphaFold DB: A0A829L2T9