Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | prlF-yhaV (relBE)/YhaV-PrlF |
| Location | 3963715..3964514 | Replicon | chromosome |
| Accession | NZ_CP069957 | ||
| Organism | Escherichia coli strain FDAARGOS_1279 | ||
Toxin (Protein)
| Gene name | yhaV | Uniprot ID | D3GWU5 |
| Locus tag | I6K09_RS18980 | Protein ID | WP_000347272.1 |
| Coordinates | 3963715..3964179 (-) | Length | 155 a.a. |
Antitoxin (Protein)
| Gene name | prlF | Uniprot ID | S1EB98 |
| Locus tag | I6K09_RS18985 | Protein ID | WP_001307405.1 |
| Coordinates | 3964179..3964514 (-) | Length | 112 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| I6K09_RS18950 (3958716) | 3958716..3959150 | - | 435 | WP_000948824.1 | PTS galactosamine/N-acetylgalactosamine transporter subunit IIA | - |
| I6K09_RS18955 (3959168) | 3959168..3960046 | - | 879 | WP_001298314.1 | PTS N-acetylgalactosamine transporter subunit IID | - |
| I6K09_RS18960 (3960036) | 3960036..3960815 | - | 780 | WP_000406214.1 | PTS N-acetylgalactosamine transporter subunit IIC | - |
| I6K09_RS18965 (3960826) | 3960826..3961299 | - | 474 | WP_001295547.1 | PTS N-acetylgalactosamine transporter subunit IIB | - |
| I6K09_RS18970 (3961322) | 3961322..3962602 | - | 1281 | WP_001521382.1 | tagatose-bisphosphate aldolase subunit KbaZ | - |
| I6K09_RS18975 (3962851) | 3962851..3963660 | + | 810 | WP_000072171.1 | aga operon transcriptional regulator AgaR | - |
| I6K09_RS18980 (3963715) | 3963715..3964179 | - | 465 | WP_000347272.1 | type II toxin-antitoxin system ribonuclease toxin YhaV | Toxin |
| I6K09_RS18985 (3964179) | 3964179..3964514 | - | 336 | WP_001307405.1 | type II toxin-antitoxin system antitoxin PrlF | Antitoxin |
| I6K09_RS18990 (3964663) | 3964663..3966234 | - | 1572 | WP_001273738.1 | galactarate dehydratase | - |
| I6K09_RS18995 (3966609) | 3966609..3967943 | + | 1335 | WP_000599651.1 | galactarate/glucarate/glycerate transporter GarP | - |
| I6K09_RS19000 (3967959) | 3967959..3968729 | + | 771 | WP_001058227.1 | 2-dehydro-3-deoxyglucarate aldolase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 3963715..3975166 | 11451 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 155 a.a. Molecular weight: 17832.26 Da Isoelectric Point: 9.6924
>T192303 WP_000347272.1 NZ_CP069957:c3964179-3963715 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH
Download Length: 465 bp
>T192303 NZ_CP092288:1044611-1044913 [Marinobacter sp. M2C]
ATGGCTAATTCCCTGGACAAACTTTTGGCGAGTGAGAAGCCGGAAGTCATAAAACGAGCGCAGGTTAAATCGGAGAATAT
TCTGTTAGAAATTCGTTTGAACGAAGTGCGCGCCTTATTGCAGAAAACACAAAAAGAGATGGCAAAGGCTATGGGCGTGA
CGCAGCCTACGATTGCAAGCATGGAGAAAATTGGAAAAGACTTGAAGCTGTCTTCCATTAAACGGTATGTGGAAAGCGTC
GGCGCGAAGGTTCGTCTGGATATTGAGCTTCCGGACGGAACCCACCATGCTATTCCGCTGTGA
ATGGCTAATTCCCTGGACAAACTTTTGGCGAGTGAGAAGCCGGAAGTCATAAAACGAGCGCAGGTTAAATCGGAGAATAT
TCTGTTAGAAATTCGTTTGAACGAAGTGCGCGCCTTATTGCAGAAAACACAAAAAGAGATGGCAAAGGCTATGGGCGTGA
CGCAGCCTACGATTGCAAGCATGGAGAAAATTGGAAAAGACTTGAAGCTGTCTTCCATTAAACGGTATGTGGAAAGCGTC
GGCGCGAAGGTTCGTCTGGATATTGAGCTTCCGGACGGAACCCACCATGCTATTCCGCTGTGA
Antitoxin
Download Length: 112 a.a. Molecular weight: 12358.94 Da Isoelectric Point: 4.8616
>AT192303 WP_001307405.1 NZ_CP069957:c3964514-3964179 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE
Download Length: 336 bp
>AT192303 NZ_CP092288:1045031-1045411 [Marinobacter sp. M2C]
ATGAGCGCACAAATTATCGAACGCGAAGGAAAGCCGGAGTATGCCGTATTGCCTTACCATGAGTACGTGCAGTTGATGGA
GCTGGCTGAAAGTGTGCGGGATGCGAGAGACGCAAGAGAAGCTATGCGTGAGCTCGACAGCGGCGAGGACGAAGCCATCC
CCGCCAACATCGTTGGCCGTTTGATCGCCGGAGACGACCACCCGCTGAAAATCTGGCGGGAGTATCGCAAGCTTACCCAA
GCAGCTTTGGGTAATGAAGCCGGGGTGGGCAAGTCCTATATCAGCCAGATTGAAACCGGCAGCAAAACTGGCTCAGCCAA
AGTGCTGAAGGCGCTGGCGGAAGCTCTGCAGGTGGATATGGATGATCTGTTGGTAGAGTGA
ATGAGCGCACAAATTATCGAACGCGAAGGAAAGCCGGAGTATGCCGTATTGCCTTACCATGAGTACGTGCAGTTGATGGA
GCTGGCTGAAAGTGTGCGGGATGCGAGAGACGCAAGAGAAGCTATGCGTGAGCTCGACAGCGGCGAGGACGAAGCCATCC
CCGCCAACATCGTTGGCCGTTTGATCGCCGGAGACGACCACCCGCTGAAAATCTGGCGGGAGTATCGCAAGCTTACCCAA
GCAGCTTTGGGTAATGAAGCCGGGGTGGGCAAGTCCTATATCAGCCAGATTGAAACCGGCAGCAAAACTGGCTCAGCCAA
AGTGCTGAAGGCGCTGGCGGAAGCTCTGCAGGTGGATATGGATGATCTGTTGGTAGAGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829KUD2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0XVC7 |