Detailed information of TA system TA192302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3129870..3130472
• Replicon: chromosome
• Accession: NZ_CP069957
• Organism: Escherichia coli strain FDAARGOS_1279

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: I6K09_RS15060
• Protein ID: WP_000897305.1
• Coordinates: 3130161..3130472 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K09_RS15055
• Protein ID: WP_000356395.1
• Coordinates: 3129870..3130160 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K09_RS15020 (3125493)	3125493..3126395	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
I6K09_RS15025 (3126392)	3126392..3127027	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K09_RS15030 (3127024)	3127024..3127953	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
I6K09_RS15035 (3128135)	3128135..3128377	-	243	WP_021523315.1	hypothetical protein	-
I6K09_RS15040 (3128596)	3128596..3128814	-	219	WP_001295676.1	CopG family transcriptional regulator	-
I6K09_RS15045 (3129233)	3129233..3129511	-	279	WP_001306650.1	hypothetical protein	-
I6K09_RS15050 (3129573)	3129573..3129785	-	213	WP_000197769.1	hypothetical protein	-
I6K09_RS15055 (3129870)	3129870..3130160	-	291	WP_000356395.1	NadS family protein	Antitoxin
I6K09_RS15060 (3130161)	3130161..3130472	-	312	WP_000897305.1	hypothetical protein	Toxin
I6K09_RS15065 (3130701)	3130701..3131609	+	909	WP_001306651.1	alpha/beta hydrolase	-
I6K09_RS25975 (3131778)	3131778..3132692	-	915	WP_077634137.1	transposase	-
I6K09_RS25980 (3132705)	3132705..3133592	-	888	Protein_2973	hypothetical protein	-
I6K09_RS15075 (3134008)	3134008..3134949	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K09_RS15080 (3134994)	3134994..3135431	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T192302 WP_000897305.1 NZ_CP069957:c3130472-3130161 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T192302 NZ_CP092288:c1042912-1042730 [Marinobacter sp. M2C]
GTGACGAGCGATGAGGTTAAAAAGAAACTGGAAAAAGATGGCTGGGAGTTGGTGCGAGTGAATGGGAGCCATCATCACTT
TAAGCATTCCACAAAGCCGGGGCTGGTTACGCTGCCACACCCTAAGTCAGAACTGCCAAAGGGTACGTTGAACAATATCT
GGAAGCAGGCCGGTTGGAAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT192302 WP_000356395.1 NZ_CP069957:c3130160-3129870 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT192302 NZ_CP092288:c1042689-1042258 [Marinobacter sp. M2C]
ATGCGTTTCCCAGTAGCACTTCATACAGATGATGGAAAGAACTACGGCGTTACTGTGCCCGATATTCCTGGCTGCTTCTC
TGCAGGCAGCAACGAAGACAAGGCTTTAGACAACGCACGTGAGGCAATTCTTGGTCACCTTGAGCTATTAGCAGAATTTG
GAGAAGACATTCCTACTGCCAACTCTATTGCCGAGCACCGTACAAACGCAGACTTTGAGGGTGCGGTGTGGGGCTTTATC
GAGATCGATGTAACACCACTCCTGGGCAAGGCAGAGAAGATCAATATCACCGTGCCCCGTTTGGTTTTGCGTCAGATTGA
CAGATATGTGAAGGAGCACCCGAACGAGGCGCGGTCACGCTCTGCGTTTCTATCTGAGGCAGCTTTGGAAAGGATGCGTA
AATCGCACGACAAGGCTAAAACCCTGGCATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7