Detailed information of TA system TA192296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2159908..2160602
• Replicon: chromosome
• Accession: NZ_CP069957
• Organism: Escherichia coli strain FDAARGOS_1279

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: I6K09_RS10470
• Protein ID: WP_001521903.1
• Coordinates: 2159908..2160306 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6K09_RS10475
• Protein ID: WP_000554758.1
• Coordinates: 2160309..2160602 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (2155738)	2155738..2155818	-	81	NuclAT_11	-	-
- (2155738)	2155738..2155818	-	81	NuclAT_11	-	-
- (2155738)	2155738..2155818	-	81	NuclAT_11	-	-
- (2155738)	2155738..2155818	-	81	NuclAT_11	-	-
I6K09_RS10440 (2155078)	2155078..2156322	-	1245	WP_000189541.1	esterase FrsA	-
I6K09_RS10445 (2156414)	2156414..2156872	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
I6K09_RS10450 (2157133)	2157133..2158590	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
I6K09_RS10455 (2158647)	2158647..2158999	-	353	Protein_2066	peptide chain release factor H	-
I6K09_RS10460 (2158995)	2158995..2159201	-	207	Protein_2067	RtcB family protein	-
I6K09_RS10465 (2159446)	2159446..2159898	-	453	WP_023144376.1	GNAT family N-acetyltransferase	-
I6K09_RS10470 (2159908)	2159908..2160306	-	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6K09_RS10475 (2160309)	2160309..2160602	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6K09_RS10480 (2160654)	2160654..2161709	-	1056	WP_001226177.1	DNA polymerase IV	-
I6K09_RS10485 (2161780)	2161780..2162565	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
I6K09_RS10490 (2162537)	2162537..2164249	+	1713	Protein_2073	flagellar biosynthesis protein FlhA	-
I6K09_RS10495 (2164328)	2164328..2164681	+	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
I6K09_RS10500 (2164738)	2164738..2165487	-	750	WP_016233951.1	C40 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2149845..2160602	10757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T192296 WP_001521903.1 NZ_CP069957:c2160306-2159908 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T192296 NZ_CP092284:c2615352-2615179 [Marinobacter sp. M3C]
ATGGACGAGTTTGAGTTCGACGAAACTAAAAGTCAGACCAATCTGGATAAGCACGGCATTGATTTCGTGGCCGCCCGAGT
TCTGTGGAAAGATCCGTATCTATTGGAAATCCGCGCAAAATCAGAGGGCGAGCCAAGATTCGTGTTGATAGGCAAGATTG
GGGAAAGACACTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT192296 WP_000554758.1 NZ_CP069957:c2160602-2160309 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT192296 NZ_CP092284:c2615099-2614845 [Marinobacter sp. M3C]
ATGAAAGCTAGAGACTTCGATAAAAAATTCGAGGAAGGTCAGGACGATATTGTTGATGACCTAGATCTGTCCACGGTCCG
CCGTGTCAATCAGGAACAAAAGCGAATCAACGTGGACTTTCCTGCTTGGGTCGTAGAGTCACTGGATCGTGAGGCGGCGC
GCATTGGTGTTACCCGCCAGTCAATCATCAAGGTGTGGCTGGTTGAGCGACTTCAAGCAGAGTCTGCTAACAAGTGCGTC
ACTCGGACGCACTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3