Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4163528..4164327 Replicon chromosome
Accession NZ_CP069945
Organism Escherichia coli strain FDAARGOS_1373

Toxin (Protein)


Gene name yhaV Uniprot ID A0A793TU43
Locus tag I6L03_RS19720 Protein ID WP_001764907.1
Coordinates 4163863..4164327 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag I6L03_RS19715 Protein ID WP_001296435.1
Coordinates 4163528..4163863 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L03_RS19700 (4159313) 4159313..4160083 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
I6L03_RS19705 (4160099) 4160099..4161433 - 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
I6L03_RS19710 (4161808) 4161808..4163379 + 1572 WP_001273763.1 galactarate dehydratase -
I6L03_RS19715 (4163528) 4163528..4163863 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
I6L03_RS19720 (4163863) 4163863..4164327 + 465 WP_001764907.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
I6L03_RS19725 (4164382) 4164382..4165191 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
I6L03_RS19730 (4165440) 4165440..4166720 + 1281 WP_001764906.1 tagatose-bisphosphate aldolase subunit KbaZ -
I6L03_RS19735 (4166743) 4166743..4167216 + 474 WP_001298322.1 PTS N-acetylgalactosamine transporter subunit IIB -
I6L03_RS19740 (4167227) 4167227..4168006 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
I6L03_RS19745 (4167996) 4167996..4168874 + 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
I6L03_RS19750 (4168892) 4168892..4169326 + 435 WP_000948834.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17705.08 Da        Isoelectric Point: 9.6927

>T192246 WP_001764907.1 NZ_CP069945:4163863-4164327 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSGKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T192246 NZ_CP092252:2763234-2763341 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT192246 WP_001296435.1 NZ_CP069945:4163528-4163863 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT192246 NZ_CP092252:c2763184-2763121 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A793TU43

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References