Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2341961..2342523 Replicon chromosome
Accession NZ_CP069945
Organism Escherichia coli strain FDAARGOS_1373

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I6L03_RS11020 Protein ID WP_000605675.1
Coordinates 2342245..2342523 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6L03_RS11015 Protein ID WP_000781370.1
Coordinates 2341961..2342245 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L03_RS11000 (2338766) 2338766..2339650 + 885 WP_001764521.1 formate dehydrogenase N subunit beta -
I6L03_RS11005 (2339643) 2339643..2340296 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
I6L03_RS11015 (2341961) 2341961..2342245 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
I6L03_RS11020 (2342245) 2342245..2342523 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6L03_RS11025 (2342709) 2342709..2343719 - 1011 WP_000642413.1 alcohol dehydrogenase AdhP -
I6L03_RS11030 (2343853) 2343853..2345550 - 1698 WP_000433460.1 malate dehydrogenase -
I6L03_RS11035 (2345707) 2345707..2345844 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6L03_RS11040 (2345946) 2345946..2346161 - 216 WP_000495766.1 biofilm-dependent modulation protein -
I6L03_RS11045 (2346506) 2346506..2346937 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2331805..2342789 10984


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T192231 WP_000605675.1 NZ_CP069945:c2342523-2342245 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T192231 NZ_CP092206:4118398-4118739 [Leptospira weilii]
ATGATTCGTGGTGAAATTTGGTGGGTAGACTTGGGAATTCCCTTTGGAAGTGAGCCAGGATTCAAACGTCCGGTTTTGAT
AATTCAAGATGACTCTTTTAATCAAAGTAATATTAATACAATAGTTTCGATTGCAATTACATCGAATTTAAATCTATCCG
AAGCACCAGGTAACGTTTTAATTAGTAAGAAAGATTCGAATTTATCAAAGGATTCAGTCGTAAATGTCTCTCAAATTGTG
ACTTTAGATAAAAAAAGATTTCTTAATAAAGTAGGAAAACTTAAATCCAATAAACTAAATGAAGTTGAAGCGGGGCTAAA
GTTAGTTACAGACTTAGATTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT192231 WP_000781370.1 NZ_CP069945:c2342245-2341961 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT192231 NZ_CP092206:4118163-4118411 [Leptospira weilii]
ATGAAAACGGCAATCTCAATTCCTGATGAGCTATTTAAGACCGCAGAAAAAACAGCTAAAAAGCTTGGTATTCCTCGTAG
TCAGTTATTTGCAAAAGCATTAGAGGAATTTATTCAATCTCATAGTAAAGAATCCGTAACTGAGAAATTAAATAAAGTCT
ACAATAGTAAATCTAGCGAAACTAAAAATAACATCACTGACTTATCCGTAGAATCACTTCGCAAAAGTTTAAAAAATGAT
TCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References