Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1363147..1363826 Replicon chromosome
Accession NZ_CP069945
Organism Escherichia coli strain FDAARGOS_1373

Toxin (Protein)


Gene name higB Uniprot ID A0A777RS26
Locus tag I6L03_RS06270 Protein ID WP_001764312.1
Coordinates 1363147..1363449 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag I6L03_RS06275 Protein ID WP_000806442.1
Coordinates 1363485..1363826 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L03_RS06250 (1358410) 1358410..1359630 - 1221 WP_001764310.1 fosmidomycin MFS transporter -
I6L03_RS06255 (1359848) 1359848..1361500 + 1653 WP_032286904.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
I6L03_RS06260 (1361537) 1361537..1362016 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
I6L03_RS06265 (1362220) 1362220..1363014 - 795 WP_000365160.1 TraB/GumN family protein -
I6L03_RS06270 (1363147) 1363147..1363449 + 303 WP_001764312.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6L03_RS06275 (1363485) 1363485..1363826 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
I6L03_RS06280 (1363884) 1363884..1366388 - 2505 WP_001468080.1 copper-exporting P-type ATPase CopA -
I6L03_RS06285 (1366650) 1366650..1367582 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.3980

>T192229 WP_001764312.1 NZ_CP069945:1363147-1363449 [Escherichia coli]
MTQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T192229 NZ_CP092206:71758-72159 [Leptospira weilii]
ATGACTCAATACTTGTTGGATACGAATATCTGTATCTATATAATTAATCAAAAGCCGGAGTCTGTTTATAAGAAATTTAA
AAAGATTAAATTAGAGAATATTTTCATTTCATCAATTACGGAATTTGAATTAAAATACGGTGTTCAGAAGAGTCTTCATT
TTGAAAGAAATCTGAAAGTCTTAGAAGAATTTTTAAGTTATTTGAATATTCTTCCGTTTGTTAGTGAAGATACGAATAAA
GCTGCAAAAGTTCGAGTAGAATTAGAAAGAGTAGGGAAACCAATTGGTCCTTTCGATCTTCTAATCGCTTCTCAGGCATT
ATCGAATAAACTTACGCTTGTGACAAACAATGAAAAAGAATTTACTCGGATTAAAGAAATTAAAATTGAAAACTGGCTTT
AA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT192229 WP_000806442.1 NZ_CP069945:1363485-1363826 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT192229 NZ_CP092206:71531-71761 [Leptospira weilii]
ATGAATAAAGCTAAAATTTTTAAAAATGGTGACAGCCAAGCAATACGTTTACCAAAGGACTATCGTTTTAAGGGTAAAGA
AGTTTATATACGAAAAGATGGCGATAACGTCATTTTAACGCCAATAGACGATGCGGTAGATCGATTTTGGAATACGGTTA
ATAATTTTTCTGAAGATCTGCTTATTGATCGGAATCAACCAAATGGCTACGATAAACGAAATAGAATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A777RS26

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References