Detailed information of TA system TA192221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 145316..145918
• Replicon: chromosome
• Accession: NZ_CP069945
• Organism: Escherichia coli strain FDAARGOS_1373

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6L03_RS00685
• Protein ID: WP_000897302.1
• Coordinates: 145316..145627 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6L03_RS00690
• Protein ID: WP_000356397.1
• Coordinates: 145628..145918 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L03_RS00655 (140346)	140346..141131	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
I6L03_RS00660 (141230)	141230..141829	+	600	WP_001314324.1	glucose-1-phosphatase	-
I6L03_RS00665 (141823)	141823..142695	+	873	WP_000920762.1	virulence factor BrkB family protein	-
I6L03_RS00670 (142692)	142692..143129	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
I6L03_RS00675 (143174)	143174..144115	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6L03_RS00680 (144179)	144179..145087	-	909	WP_001331553.1	alpha/beta hydrolase	-
I6L03_RS00685 (145316)	145316..145627	+	312	WP_000897302.1	hypothetical protein	Toxin
I6L03_RS00690 (145628)	145628..145918	+	291	WP_000356397.1	NadS family protein	Antitoxin
I6L03_RS00695 (146277)	146277..146555	+	279	WP_001296612.1	hypothetical protein	-
I6L03_RS00700 (146951)	146951..147169	+	219	WP_001314326.1	CopG family transcriptional regulator	-
I6L03_RS00705 (147393)	147393..148322	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6L03_RS00710 (148319)	148319..148954	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6L03_RS00715 (148951)	148951..149853	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T192221 WP_000897302.1 NZ_CP069945:145316-145627 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T192221 NZ_CP092185:c3560758-3560408 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT192221 WP_000356397.1 NZ_CP069945:145628-145918 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT192221 NZ_CP092185:c3561045-3560764 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: S1P416