Detailed information of TA system TA192204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 100023..100750
• Replicon: plasmid unnamed
• Accession: NZ_CP069941
• Organism: Klebsiella pneumoniae strain FDAARGOS_1312

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: I6K42_RS25955
• Protein ID: WP_011251285.1
• Coordinates: 100439..100750 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K42_RS25950
• Protein ID: WP_011251286.1
• Coordinates: 100023..100442 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K42_RS25920	95191..95661	-	471	WP_048333570.1	hypothetical protein	-
I6K42_RS25925	95854..96609	-	756	Protein_96	hypothetical protein	-
I6K42_RS25930	97205..97648	+	444	WP_048333569.1	hypothetical protein	-
I6K42_RS25935	97669..98457	+	789	WP_040217257.1	hypothetical protein	-
I6K42_RS25940	98471..98806	+	336	Protein_99	conjugal transfer protein TraI	-
I6K42_RS25945	98908..99876	-	969	WP_074428168.1	IS5 family transposase	-
I6K42_RS25950	100023..100442	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
I6K42_RS25955	100439..100750	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6K42_RS25960	100955..101413	-	459	Protein_103	DDE-type integrase/transposase/recombinase	-
I6K42_RS25965	101527..102224	+	698	Protein_104	IS1 family transposase	-
I6K42_RS25970	102241..102675	+	435	Protein_105	thioredoxin fold domain-containing protein	-
I6K42_RS25975	102692..103003	+	312	WP_011251282.1	hypothetical protein	-
I6K42_RS25980	103017..103358	+	342	WP_011251281.1	hypothetical protein	-
I6K42_RS25985	103418..104374	+	957	WP_011251280.1	DsbA family protein	-
I6K42_RS25990	104491..105066	+	576	WP_074424927.1	hypothetical protein	-
I6K42_RS25995	105174..105689	+	516	WP_041937820.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..224781	224781
	-	inside	IScluster/Tn	-	-	89902..102224	12322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T192204 WP_011251285.1 NZ_CP069941:c100750-100439 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T192204 NZ_CP092184:c323697-322744 [Serratia marcescens]
ATGGATGACCTCACCACTCTTTTTTCTGAGGTAGCGGATGCACTCGGCATTGAGTCTGTCGCTATTGTAGAAAAAGATCA
CTATATCCTTGAGCTTCTGGCTCTGCTTAAGCCATTGTCATTTTGTACTCATCAACTGGTATTCGCAGGCGGTACCGCCC
TGGCAAAATCCGGGGTGTTGCTGAACCGCATGTCAGAAGATGTGGATATCAAAATGGTTCCAACTGCTGCCTTTCTGACG
CTTTCGCGCACCAGGCGAAAAACCAGCCGTAAAGCGGTTATTCAGTCAATCATCGACGCAATAACCGCTACCGATATGTT
CAGTGTTGATGATGGCCAGCAAGTGACCCGTGATGAGTATCGCTATAATGATATTCCGGTTCGCTATCCTCAGCGTCTGG
CACAGGAGCCCTGCCTGCGCCCTTTCATCAAACTGGAGCTGATGGAGGCTGATTTGCTGGAACCCGCAGAAGCACGGGAT
ATTAACTCACTGGCAACCGGGTTGCTGAAAAAGCCACCAGTCGTTCAGGCCTTTCCTTCCGTTACCGTAACCAGTACACA
AGCTGAGAAATTAGTGGCGATGTTGCGACGGACAGCCGCCGTCATTCGCAATGTTGAACGTTTTGATGATACCACGTTGG
TTCGCCATATCTACGATACCTGGTGCATTATGCACGCCAGTCATGTCGATATGGCACTGCTGCCTGTTTTTGTTGAGAGA
ACCATCAATCAGGATATCCAGCGTTACGGTAACCAGTACCCACAATTTTGCGACTCACCTGTCGCAGAGCTGAAACTGGG
GCTGGAGGAACTGGAGAACAATCCTGTTCATAAGGAACGTTATCAGCAATTTGTTGCACCAATGGTATTTGGCAAACAAG
TCACCTGGGTAGAGGCTTATGGCTGCTTCAGGCAAGTGGCACTTTCCATTCTAGACACTCTGCCTGCAAGGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT192204 WP_011251286.1 NZ_CP069941:c100442-100023 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT192204 NZ_CP092184:c324085-323690 [Serratia marcescens]
ATGACGACCAAAGCCCGTATCCAGTCACGACTGAAACGCTCGAAGCGCTATGTTTTCACTCGCGATGATTTCAAAGATAT
TGCTGGCTACGATCAGATTGGCAGAGCGTTGCGAGCCCTGGTTAAAGAAGGTGAACTGTTGAAAGTCGGTTATGGCGTGT
ACACCAAAGCACGTCGCAACAGTATTACCGGTAGGATAATGCCAGCATCGCCCGGTGGTTCCGCTGCGGTGATCGTCGAA
ACGCTGGATCGGCTGAAGGTACGCTATCGTTTTACCGATGCGACACTGGCCTATAACAGCGGTCAGTCTACACAGGTCCC
TGTTTCGGCAGGGATAAAAACACCATCGCGTTTTAAACGTGTGTTGTCTGTAGGGAACAGCAAACTGAATGGATGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1