Detailed information of TA system TA192168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4909737..4910431
• Replicon: chromosome
• Accession: NZ_CP069935
• Organism: Escherichia coli strain FDAARGOS_1302

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: I6K32_RS23750
• Protein ID: WP_001263500.1
• Coordinates: 4910033..4910431 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6K32_RS23745
• Protein ID: WP_000554758.1
• Coordinates: 4909737..4910030 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K32_RS23720 (4905273)	4905273..4905770	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
I6K32_RS23725 (4905853)	4905853..4906011	-	159	WP_014639450.1	hypothetical protein	-
I6K32_RS23730 (4906090)	4906090..4907802	-	1713	Protein_4677	flagellar biosynthesis protein FlhA	-
I6K32_RS23735 (4907774)	4907774..4908559	+	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
I6K32_RS23740 (4908630)	4908630..4909685	+	1056	WP_001226168.1	DNA polymerase IV	-
I6K32_RS23745 (4909737)	4909737..4910030	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6K32_RS23750 (4910033)	4910033..4910431	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6K32_RS23755 (4910441)	4910441..4910893	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
I6K32_RS23760 (4911083)	4911083..4912222	+	1140	WP_000521577.1	RNA ligase RtcB family protein	-
I6K32_RS23765 (4912219)	4912219..4912833	+	615	WP_000602123.1	peptide chain release factor H	-
I6K32_RS23770 (4912890)	4912890..4914347	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
I6K32_RS23775 (4914608)	4914608..4915066	+	459	WP_001291988.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	vgrG/tssI / gmhA/lpcA	4890213..4910893	20680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T192168 WP_001263500.1 NZ_CP069935:4910033-4910431 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T192168 NZ_CP092112:c738943-738683 [Leptospira noguchii]
GTGTCAAAAAGTGAAAAATTAATCCAAAGGTTCTTAGGACGACCTAAAGATTTTTCTTACGATGAACTTAGAAAATTATT
TTCAGGCTTTGGGTATATTGAAGATAATTCAGGTAAATCTTCTGGCTCAAGAGTTGCTTGGATTCATTCGGAGACGAAAC
ATATTATTAGACTTCATAAACCTCATCCGAAGAATATTTTGAAGTCATATCAGATAAATCAAATTTTAGATGAATTAAAA
TCGGAGGGCTATATAAAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT192168 WP_000554758.1 NZ_CP069935:4909737-4910030 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT192168 NZ_CP092112:c738686-738330 [Leptospira noguchii]
ATGAAAGATATTATCGAATATAACGGCTTTTTAGGTTCTGTTCATTTTGATGCAGACGATGAAGTATTTTTTGGAAAAAT
AGAAGGAATTGAAGATTTGGTTTCCTTTGAAGGTGAATCAGTTAAACAACTGAAGAAGGCATTTCAAGAATCAGTAGATG
ATTATTTAATCTTATGCAAAAAGGCTAAAAAGAGTCCAGAGAAATCTTTTAAAGGTTCATTTAATGTTCGCATACCAACT
GAACTTCATCGACGCGTTTATAGAAAATCGTTAAGAGAAGGCATTTCTTTGAATCAATTAGTTCAGAGAGCTTTAGAAAA
AGAAATCACGGAACTTAAGACGCCACACTTCGGCTAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3