Detailed information of TA system TA192159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3938506..3939108
• Replicon: chromosome
• Accession: NZ_CP069935
• Organism: Escherichia coli strain FDAARGOS_1302

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6K32_RS19160
• Protein ID: WP_000897302.1
• Coordinates: 3938506..3938817 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K32_RS19165
• Protein ID: WP_000356397.1
• Coordinates: 3938818..3939108 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K32_RS19135 (3934420)	3934420..3935019	+	600	WP_001314324.1	glucose-1-phosphatase	-
I6K32_RS19140 (3935013)	3935013..3935885	+	873	WP_000920762.1	virulence factor BrkB family protein	-
I6K32_RS19145 (3935882)	3935882..3936319	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
I6K32_RS19150 (3936364)	3936364..3937305	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K32_RS19155 (3937369)	3937369..3938277	-	909	WP_001331553.1	alpha/beta hydrolase	-
I6K32_RS19160 (3938506)	3938506..3938817	+	312	WP_000897302.1	hypothetical protein	Toxin
I6K32_RS19165 (3938818)	3938818..3939108	+	291	WP_000356397.1	NadS family protein	Antitoxin
I6K32_RS19170 (3939467)	3939467..3939745	+	279	WP_001296612.1	hypothetical protein	-
I6K32_RS19175 (3940141)	3940141..3940359	+	219	WP_001314326.1	CopG family transcriptional regulator	-
I6K32_RS19180 (3940575)	3940575..3941504	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6K32_RS19185 (3941501)	3941501..3942136	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K32_RS19190 (3942133)	3942133..3943035	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T192159 WP_000897302.1 NZ_CP069935:3938506-3938817 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T192159 NZ_CP092107:c301126-300839 [Vibrio sp. JC009]
ATGAAGGTAGTTTGGTCCCCTTTGGCATTACAAAAATTAGGCGATGCAGCCGAATTTATCTCATTGGACAATCCATCAGC
AGCCAGGAAGTGGGTGGATGAAGTCTTTGATAAAGCTGAATTACTTGCTTCGATGCCAGAGATGGGGCGTATGGTTCCTG
AAATGCCGAATACAAACTATCGGGAAGTTATATTCGGTCATTACAGGATCATTTACAGCATAAGCCATGAGATTCGGGTA
TTAACTGTTCGTAATTGTCGCCAAATTCTTACGGATGATGCTCTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT192159 WP_000356397.1 NZ_CP069935:3938818-3939108 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT192159 NZ_CP092107:c301404-301123 [Vibrio sp. JC009]
ATGAGCCGTATACATTTCGATAAAGACATACAACCGTTGTCAGAATTTCGCGCTGGCGTAACCTCATTTATAAAGCAAAT
AAACGAAACTCGACGTCCATTGGTAATCACTCAACGTGGTAAAGGTGTTGCAGTGGTACTTGATGTCGCTGAATATGAAG
CCATGCAAGAGAAAATCGAGCTATTGGAAGAAATGCGTACCGCTGAAGCTCAATTAGCATCTGGCCTGAGTGTTTCTAAT
GAAGATGCACGAGCTCAAGTATTAGGACGTATTAAAGAATGA

Structures

Toxin

• AlphaFold DB: S1P416