Detailed information of TA system TA192130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 5197989..5198586
• Replicon: chromosome
• Accession: NZ_CP069907
• Organism: Klebsiella pneumoniae strain FDAARGOS_1313

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: I6K43_RS28410
• Protein ID: WP_004893639.1
• Coordinates: 5197989..5198306 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: I6K43_RS28415
• Protein ID: WP_004142561.1
• Coordinates: 5198299..5198586 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K43_RS28395	5193693..5195120	-	1428	WP_009308097.1	MFS transporter	-
I6K43_RS28400	5195229..5196095	+	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
I6K43_RS28405	5196761..5197804	+	1044	WP_009308098.1	DUF2157 domain-containing protein	-
I6K43_RS28410	5197989..5198306	+	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K43_RS28415	5198299..5198586	+	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
I6K43_RS28420	5198851..5199504	-	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
I6K43_RS28425	5199624..5199992	-	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
I6K43_RS28430	5199992..5200573	-	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
I6K43_RS28435	5200753..5201868	+	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
I6K43_RS28440	5201899..5202240	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
I6K43_RS28445	5202258..5202506	-	249	WP_002893037.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T192130 WP_004893639.1 NZ_CP069907:5197989-5198306 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T192130 NZ_CP092077:327901-328269 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT192130 WP_004142561.1 NZ_CP069907:5198299-5198586 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT192130 NZ_CP092077:327683-327904 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1