Detailed information of TA system TA192097

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 85353..85607
• Replicon: plasmid unnamed1
• Accession: NZ_CP069894
• Organism: Escherichia coli strain FDAARGOS_1286

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: I6K16_RS24655
• Protein ID: WP_001312851.1
• Coordinates: 85458..85607 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	85353..85414 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K16_RS24630 (82101)	82101..82847	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
I6K16_RS24635 (82906)	82906..83766	+	861	WP_000704513.1	alpha/beta hydrolase	-
I6K16_RS24640 (83869)	83869..84429	+	561	WP_000139315.1	fertility inhibition protein FinO	-
I6K16_RS24645 (84565)	84565..84777	+	213	WP_001299730.1	ANR family transcriptional regulator	-
I6K16_RS24650 (85023)	85023..85097	+	75	Protein_97	endonuclease	-
- (85353)	85353..85414	-	62	NuclAT_1	-	Antitoxin
- (85353)	85353..85414	-	62	NuclAT_1	-	Antitoxin
- (85353)	85353..85414	-	62	NuclAT_1	-	Antitoxin
- (85353)	85353..85414	-	62	NuclAT_1	-	Antitoxin
I6K16_RS24655 (85458)	85458..85607	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
I6K16_RS24660 (85891)	85891..86148	+	258	WP_000083845.1	replication regulatory protein RepA	-
I6K16_RS25455 (86165)	86165..86416	-	252	WP_223195197.1	replication protein RepA	-
I6K16_RS25460 (86407)	86407..86454	+	48	WP_229471593.1	hypothetical protein	-
I6K16_RS24670 (86447)	86447..86929	+	483	WP_001273588.1	hypothetical protein	-
I6K16_RS24675 (86922)	86922..87779	+	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
I6K16_RS24680 (88742)	88742..88993	+	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
I6K16_RS24685 (88990)	88990..89277	+	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iucA / iucB / iucC / iucD / iutA / vat / iroN / iroE / iroD / iroC / iroB	1..153221	153221

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T192097 WP_001312851.1 NZ_CP069894:85458-85607 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T192097 NZ_CP092070:1144324-1144638 [Pectobacterium sp. PL64]
ATGCAATTCACTGTATACGGTAATACCGGGAAAAGCGTCGTTTACCCGCTGTTGCTCGACGTCACGAGCGATATTATTGG
GCAATTGAATCGTCGGATAGTGATCCCATTGCTCCCTATTGAAAAATATCCGGCAGGCCGCCGACCGGATCGCCTTGTCC
CCCTCATCAGGCTGACGGACGGCAAAGAATACGCCGTAATGACCCACGAACTGGCAAGTATCCCTGTCCAGGCTCTGGGG
GCGGTGTTTTGTGATGCGTCGCAGTACCGCACTCAGGTAAAGGCGGCAATAGACTTCCTTATCGACGGATTCTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT192097 NZ_CP069894:c85414-85353 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin