Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3851373..3852052 Replicon chromosome
Accession NZ_CP069893
Organism Escherichia coli strain FDAARGOS_1286

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag I6K16_RS18345 Protein ID WP_000057523.1
Coordinates 3851373..3851675 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag I6K16_RS18350 Protein ID WP_000806442.1
Coordinates 3851711..3852052 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K16_RS18325 (3846636) 3846636..3847856 - 1221 WP_001251634.1 fosmidomycin MFS transporter -
I6K16_RS18330 (3848074) 3848074..3849726 + 1653 WP_204669700.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase UshA -
I6K16_RS18335 (3849763) 3849763..3850242 - 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
I6K16_RS18340 (3850446) 3850446..3851240 - 795 WP_000365161.1 TraB/GumN family protein -
I6K16_RS18345 (3851373) 3851373..3851675 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K16_RS18350 (3851711) 3851711..3852052 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
I6K16_RS18355 (3852110) 3852110..3854614 - 2505 WP_000083999.1 copper-exporting P-type ATPase CopA -
I6K16_RS18360 (3854877) 3854877..3855809 + 933 WP_000883024.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3851373..3861456 10083


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T192088 WP_000057523.1 NZ_CP069893:3851373-3851675 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T192088 NZ_CP092065:795502-795846 [Edwardsiella ictaluri]
ATGAAGTCACTATATTGGGTTGGTAGTACAAAAAAAGACCTCCAGTCATTGCCTGAAGAAGTACAGGATATTTTCGGCTA
TGCCCTGCATCTGGCACAGGTAGGAGGTAAACACTCACAGACCAAGCCACTGAAGGGGTTTAGTGGAGCTGGCGTACTTG
AGGTAGTAGAAGACTTTCTCGGTGATACTTATCGTGCAGTGTATACCGTTAAGTTCGGTGATGCCGTCTACGTTTCGCAC
GTATTCCAGAAAAAGTCTTCATCGGGAATCGCCACGCCAAAGCTCAATATGGACAAGATCCGCGAACGGCTAAAAGCCGC
AGAAAACCATGCCAGAGGAGCATAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT192088 WP_000806442.1 NZ_CP069893:3851711-3852052 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT192088 NZ_CP092065:795848-796165 [Edwardsiella ictaluri]
ATGAGCCATGATATTGAAGTCAGCAGCGGCAACGTTTATGTCGATATTGGCAGAGATGATGCCGAGGAAATGCTGTTAAA
AGCTCAGCTTGCCACCACTATTGGAAACATCATTAAAAGCCGTAGCCTGACGCAAGAACAAGCAGCAAAACTGCTGGGGA
TGACGCAGCCCAAACTGTCCAATATGCTGCGTGGGCAGTTCCGGGGCATCAGTGAAGCCAAAATGCTGGAATGTCTGACC
CGCTTAGGGCGCGACGTGCAAATCGTGGTTGGAAAGCCGCGCCGGACGCCGGGTAGCGTGAAAGTAGTGTTAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References