Detailed information of TA system TA192084

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2693206..2693808
• Replicon: chromosome
• Accession: NZ_CP069893
• Organism: Escherichia coli strain FDAARGOS_1286

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: I6K16_RS12960
• Protein ID: WP_000897305.1
• Coordinates: 2693206..2693517 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K16_RS12965
• Protein ID: WP_000356397.1
• Coordinates: 2693518..2693808 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K16_RS12935 (2689120)	2689120..2689719	+	600	WP_001315111.1	glucose-1-phosphatase	-
I6K16_RS12940 (2689713)	2689713..2690585	+	873	WP_000920762.1	virulence factor BrkB family protein	-
I6K16_RS12945 (2690582)	2690582..2691019	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
I6K16_RS12950 (2691064)	2691064..2692005	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K16_RS12955 (2692069)	2692069..2692977	-	909	WP_001318161.1	alpha/beta hydrolase	-
I6K16_RS12960 (2693206)	2693206..2693517	+	312	WP_000897305.1	hypothetical protein	Toxin
I6K16_RS12965 (2693518)	2693518..2693808	+	291	WP_000356397.1	NadS family protein	Antitoxin
I6K16_RS12970 (2694413)	2694413..2694631	+	219	WP_001295676.1	CopG family transcriptional regulator	-
I6K16_RS12975 (2694846)	2694846..2695775	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6K16_RS12980 (2695772)	2695772..2696407	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K16_RS12985 (2696404)	2696404..2697306	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T192084 WP_000897305.1 NZ_CP069893:2693206-2693517 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T192084 NZ_CP092062:2263216-2263632 [Edwardsiella ictaluri]
GTGGCCCTTTGGCGCTGTAATGTTCACGCCTCCTGGCGTACCCAGGTGATGTTTCTGCTGGGGCATGGCGCGCTGGTATT
GGCGATCCTGTTGGCGCCCTGGCCGGAAAACTACAGCGTATTCTGGCTGATTCTGCTGGTTGCGGTGATCTTTGAGTGTA
TCCGCAGCCAGCGTCTGATTGCGCGTAACAGCGGTGAGCTACAGCGGCTGGCCCCACAGGTATGGCACTGGCAGCTGCGC
GAGTGGCGCCTGGCCCGGCGCCCCTGGGTTAGCGATCTGGGAGCGTTGCTGATCCTGCAGAGTACCCATGGCACGCCGTG
CCGCCGTCGTCTGTGGCTGGCGGCCGACAGCATGAGCCGCGAAGAGTGGGGGCAGCTGCGCCGCGCGCTGCTCGATACGG
GCGACGATCGCGTATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT192084 WP_000356397.1 NZ_CP069893:2693518-2693808 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT192084 NZ_CP092062:2262969-2263235 [Edwardsiella ictaluri]
ATGGATATCAATAATAAAGCGCGGGTTCACTGGGCGTGCCGCCGCGGAATGCGTGAGCTGGATATCGCCATTATGCCTTT
CTTTGAACATGAGTATGATGCGCTTAACGATGCCGATAAACAGACCTTTGTCCGACTGCTGTCCTGTGACGATCCTGAAC
TATTTAAATGGCTGATGAACCAGGGTAAGCCGGACGATACCGATCTGGCCCGTATGATTACGCTGATTCAGACGCGAAAT
AAAGAACGTGGCCCTTTGGCGCTGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7