Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1786574..1787373 Replicon chromosome
Accession NZ_CP069893
Organism Escherichia coli strain FDAARGOS_1286

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag I6K16_RS08585 Protein ID WP_000347251.1
Coordinates 1786909..1787373 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag I6K16_RS08580 Protein ID WP_001296435.1
Coordinates 1786574..1786909 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K16_RS08565 (1782359) 1782359..1783129 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
I6K16_RS08570 (1783145) 1783145..1784479 - 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
I6K16_RS08575 (1784854) 1784854..1786425 + 1572 WP_001273941.1 galactarate dehydratase -
I6K16_RS08580 (1786574) 1786574..1786909 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
I6K16_RS08585 (1786909) 1786909..1787373 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
I6K16_RS08590 (1787428) 1787428..1788237 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
I6K16_RS08595 (1788486) 1788486..1789766 + 1281 WP_000681938.1 tagatose-bisphosphate aldolase subunit KbaZ -
I6K16_RS08600 (1789789) 1789789..1790262 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
I6K16_RS08605 (1790273) 1790273..1791052 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
I6K16_RS08610 (1791042) 1791042..1791920 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
I6K16_RS08615 (1791938) 1791938..1792372 + 435 WP_000948823.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T192074 WP_000347251.1 NZ_CP069893:1786909-1787373 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T192074 NZ_CP092052:c2518088-2517822 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT192074 WP_001296435.1 NZ_CP069893:1786574-1786909 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT192074 NZ_CP092052:c2518345-2518088 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References