Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4882672..4883174 Replicon chromosome
Accession NZ_CP069890
Organism Escherichia coli strain FDAARGOS_1295

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag I6K25_RS23515 Protein ID WP_000767822.1
Coordinates 4882672..4882926 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I6K25_RS23520 Protein ID WP_001259255.1
Coordinates 4882923..4883174 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K25_RS23495 (4879165) 4879165..4880523 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
I6K25_RS23500 (4880513) 4880513..4880575 - 63 WP_010723108.1 membrane protein YoeI -
I6K25_RS23505 (4880790) 4880790..4881719 - 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
I6K25_RS23510 (4881765) 4881765..4882589 - 825 WP_000754733.1 SDR family oxidoreductase -
I6K25_RS23515 (4882672) 4882672..4882926 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I6K25_RS23520 (4882923) 4882923..4883174 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I6K25_RS23525 (4883457) 4883457..4883507 + 51 WP_001364200.1 his operon leader peptide -
I6K25_RS23530 (4883653) 4883653..4884552 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
I6K25_RS23535 (4884558) 4884558..4885862 + 1305 WP_001297912.1 histidinol dehydrogenase -
I6K25_RS23540 (4885859) 4885859..4886929 + 1071 WP_000108995.1 histidinol-phosphate transaminase -
I6K25_RS23545 (4886929) 4886929..4887996 + 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T192061 WP_000767822.1 NZ_CP069890:c4882926-4882672 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T192061 NZ_CP092042:c4092533-4092042 [Enterobacter cloacae]
ATGATCTCTGCCCCAGAGCCCCTTCACGCCGGTCATATTCTTACTCCATTTTGCTGTGGTGTGGATTCGATGGATAACTG
GCTGAAGCAGCGGGCGATGAAAAATCAGGTCAGTGGTGCATCCCGTACCTTTGTCTGTTGTGGCAACGATTCTAACGTTC
TGGCTTATTACTCGCTGGCATCCAGCGCGGTCATGACGAATACCGCACCCGGTCGCTTTCGTCGCAATATGCCCGATCCC
ATCCCGGTTGTCGTGCTGGGACGCCTGGCGGTAGATCAGTCTCTGCATGGGCAGGGCGTTGGTCGGGCACTGGTACGCGA
CGCCGGGCTGCGGGTGATCCAGGTGGCGGAGACCATCGGCATTCGCGGAATGCTGGTTCATGCGCTGTCGGATGAAGCGC
GGGAATTTTATCTGCGGGTGGGGTTTGAGCCATCGCCAATGGATCCGATGATATTGATGGTGACGTTGGGGGATTTGATG
GCGAGTATTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT192061 WP_001259255.1 NZ_CP069890:c4883174-4882923 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT192061 NZ_CP092042:c4092823-4092530 [Enterobacter cloacae]
ATGCCAACTGCAAACACTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAGCCTGCCGAGCGAGATCTTATCGA
TCGGGCGGCGAAAGCGAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCAGCCCGCGCTGCTGCGGAAGAGGCACTGA
TCGAACAACGCATCATAATGGCCGATCCAGAGGCTTATCAGGAATTTCTGGCTCGTCTGGATCAGGCTCCAGCACCTAAT
GCCACACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAGCGGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References