Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4263667..4264229 Replicon chromosome
Accession NZ_CP069890
Organism Escherichia coli strain FDAARGOS_1295

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I6K25_RS20555 Protein ID WP_000605675.1
Coordinates 4263951..4264229 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6K25_RS20550 Protein ID WP_000781370.1
Coordinates 4263667..4263951 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K25_RS20535 (4258848) 4258848..4261895 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
I6K25_RS20540 (4261908) 4261908..4262792 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
I6K25_RS20545 (4262785) 4262785..4263438 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
I6K25_RS20550 (4263667) 4263667..4263951 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
I6K25_RS20555 (4263951) 4263951..4264229 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K25_RS20560 (4264415) 4264415..4265425 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
I6K25_RS20565 (4265559) 4265559..4267256 - 1698 WP_000433462.1 malate dehydrogenase -
I6K25_RS20570 (4267412) 4267412..4267549 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6K25_RS20575 (4267651) 4267651..4267866 - 216 WP_000495766.1 biofilm-dependent modulation protein -
I6K25_RS20580 (4268211) 4268211..4268642 + 432 WP_000152310.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T192050 WP_000605675.1 NZ_CP069890:c4264229-4263951 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T192050 NZ_CP092041:c49057-48641 [Salmonella enterica subsp. enterica serovar Infantis]
GTGAAGAAAATGTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCCGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCACCGTATTGTGGTCTCGGCCATCACCTACTCAGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGTCACATTGAACTGGTTGACGCGTTCTGCGCCCGCCTCGATGCTATCCTGCCCTGGGACCGGGCC
GCGGTGAACGCCACCACGGAGATTAAGGTGGCGCTACGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCGATCGC
CGGGCACGCTATTGCCGCCGGCGCGGTACTGGTGACAAATAATACGCGGGAGTTTGAGCGGGTGCCGGGTCTTGTTCTGG
AAGACTGGGGAAGATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT192050 WP_000781370.1 NZ_CP069890:c4263951-4263667 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT192050 NZ_CP092041:c49284-49054 [Salmonella enterica subsp. enterica serovar Infantis]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGATGGAGTGAG
CGAGCTGGAAATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCTGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGGCTTTATGGCGGAGCGTGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References