Detailed information of TA system TA192036

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1949462..1950064
• Replicon: chromosome
• Accession: NZ_CP069890
• Organism: Escherichia coli strain FDAARGOS_1295

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6K25_RS09365
• Protein ID: WP_000897302.1
• Coordinates: 1949462..1949773 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K25_RS09370
• Protein ID: WP_000356397.1
• Coordinates: 1949774..1950064 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K25_RS09340 (1945376)	1945376..1945975	+	600	WP_001314324.1	glucose-1-phosphatase	-
I6K25_RS09345 (1945969)	1945969..1946841	+	873	WP_000920762.1	virulence factor BrkB family protein	-
I6K25_RS09350 (1946838)	1946838..1947275	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
I6K25_RS09355 (1947320)	1947320..1948261	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6K25_RS09360 (1948325)	1948325..1949233	-	909	WP_001331553.1	alpha/beta hydrolase	-
I6K25_RS09365 (1949462)	1949462..1949773	+	312	WP_000897302.1	hypothetical protein	Toxin
I6K25_RS09370 (1949774)	1949774..1950064	+	291	WP_000356397.1	NadS family protein	Antitoxin
I6K25_RS09375 (1950423)	1950423..1950701	+	279	WP_001296612.1	hypothetical protein	-
I6K25_RS09380 (1951097)	1951097..1951315	+	219	WP_001314326.1	CopG family transcriptional regulator	-
I6K25_RS09385 (1951531)	1951531..1952460	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6K25_RS09390 (1952457)	1952457..1953092	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6K25_RS09395 (1953089)	1953089..1953991	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T192036 WP_000897302.1 NZ_CP069890:1949462-1949773 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T192036 NZ_CP092039:5029072-5030391 [Pectobacterium brasiliense]
ATGGCTGCTCTCGATGTTTACATGAACGGCTATCGGGTGGGGATTCTGACCAAAACGGGCAGTGGGGCACATCATTTTTC
CTATGATACGAACTGGCTCGGATTGCTGGGGAGTCGCCCCATTTCGCTTTCTATGCCGCTACGTCATCAGCCCTATCAAG
GCGATGAAGTTTATAACTTCTTCGACAATTTGTTGCCGGATAACCCGGATATCAGAAGACGGATTGTCGCCAGGCATCAT
ACGGATTCCACCCAACCGTTTGATCTGCTCGCTAAAGTGGGGCAAGACAGTGTCGGGGCGTTGCAATTAGTGCCGCAGGG
TACACCTGTGCACGATATAAAAAAGATCGAATATAAAACCTTATCCGAGCAGCAGCTTGAAAACATACTGACCGGATATT
TGTCCGATGCCCCGCTGGGTATGATTGATACTGAAGACGATTTTCGTATTTCTATCGCAGGTGCACAGGAAAAGACGGCA
TTGCTTTATCTGGATGATCGCTGGTGCCTGCCTCTCAATGTCACACCAACTACCCACATCATCAAGCTACCGATTGGTAA
AATCGAGAGTCACTCTTACTCAATTGATATGTCTGACAGCGTTGAAAATGAGTATCTGTGTTTGCTCATTGCCAAGGCAT
TTGGGCTGCCAGTGCCGCACTGTTTTATGATCAACGCTGGCAAGATAAAGGCGTTAGCGGTGGAGCGTTTTGATCGTAAA
TACGCCTCGGATGTCAGTTGGATTATGCGGCTACCACAAGAGGATTTTTGTCAGGTATTAAACGTTCCTTCCGCACTGAA
ATATGAAAATCATGGTGGACCGGGTATTTCCGCCATTATGTCGTTTCTATTGGGTTCGGTTTATCCAGAAAGAGATCGTT
ATCGCTTTATGCAGACTCAGGTACTGTTTTGGCTATTGGCTGCAACGGATGGTCATGCGAAAAACTTTTCCCTGTTTATT
GAATCTGAGGGACGATATCGACTCACGCCTTTCTATGACATTCTCTCTATGTATCCGGCGATGGGCGGACGGGGAATTGA
CCGCAGAGAGGCCAAACTGGCGATGGGATTAACTGGCTCAAGAGGCAAGAAATATGCGATTGAGCAGATCTTTCCCCGAC
ATTTTTTCCAGACGGCCAAGGTCGTTGGGTTCGCCAGAGAATCGATGGAAAACATTCTGGCGGAGTTTGCCCAGACCGTT
GATACCGTCATGACGACTGTTGGAAGTCAACTGCCTGTGGATTTTCCTACTCATATCAGTGAGGCAATTCTGGCCGGATT
GCAGGCCAGATCGAGACGGCTCATGAGCGGATGGGAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT192036 WP_000356397.1 NZ_CP069890:1949774-1950064 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT192036 NZ_CP092039:5028803-5029072 [Pectobacterium brasiliense]
ATGATGAAGATCACCAACAGCAAGCAGCTTAGCAGCTATCTGAAAGATGTTCGTATCACGCAGAAGCTTTCACAAGGGAA
AGTGGCCAGTAAAGTGGGTATTCGCCAGGATACGGTATCCAGTTTCGAGCAACATCCTGATTCCACTAAGTTGGAAACCT
TCTTTAAAATCCTGTCGGCATTGAATTTAGAACTTACTGTTACGCCCAGGAATGTGGACACCACCAATAACGAAGCCTCT
GTTGCATCATCCTGGAAGGAAGAATGGTAA

Structures

Toxin

• AlphaFold DB: S1P416