Detailed information of TA system TA192030

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 35281..35550
• Replicon: plasmid unnamed1
• Accession: NZ_CP069883
• Organism: Escherichia coli strain FDAARGOS_1289

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: I6K19_RS24940
• Protein ID: WP_001372321.1
• Coordinates: 35425..35550 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	35281..35346 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K19_RS24920	30503..32461	+	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
I6K19_RS24925	32516..32950	+	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
I6K19_RS24930	32947..33709	+	763	Protein_40	plasmid SOS inhibition protein A	-
-	33678..33779	+	102	NuclAT_1	-	-
-	33678..33779	+	102	NuclAT_1	-	-
-	33678..33779	+	102	NuclAT_1	-	-
-	33678..33779	+	102	NuclAT_1	-	-
I6K19_RS24935	33825..35194	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	35221..35348	+	128	NuclAT_0	-	-
-	35221..35348	+	128	NuclAT_0	-	-
-	35221..35348	+	128	NuclAT_0	-	-
-	35221..35348	+	128	NuclAT_0	-	-
-	35281..35346	-	66	-	-	Antitoxin
I6K19_RS27235	35334..35483	+	150	Protein_42	plasmid maintenance protein Mok	-
I6K19_RS24940	35425..35550	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6K19_RS27240	35770..36000	+	231	WP_001426396.1	hypothetical protein	-
I6K19_RS27245	35998..36171	-	174	Protein_45	hypothetical protein	-
I6K19_RS24950	36241..36501	+	261	WP_250187944.1	single-stranded DNA-binding protein	-
I6K19_RS24955	36595..37599	-	1005	WP_204606458.1	IS110 family transposase	-
I6K19_RS24960	37850..38137	+	288	WP_000107535.1	hypothetical protein	-
I6K19_RS24965	38257..39078	+	822	WP_001234469.1	DUF932 domain-containing protein	-
I6K19_RS24970	39375..39965	-	591	WP_277996679.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / dfrA8 / tet(B)	-	1..131034	131034

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T192030 WP_001372321.1 NZ_CP069883:35425-35550 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T192030 NZ_CP092039:4349912-4350217 [Pectobacterium brasiliense]
ATGCAATTCATTGTTTATCAATACAAACGCGCCAGTCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCAAAGCGGCGTATGGTCATCCCGCTTATCGAAGCATATCACCTGTCTGAGAAAGTGAATAAAACATTGTTCCCTTTGA
TCCGCATCGACGGCGAAGATTATCGGCTGATGACAACTGAACTATCGAGCGTTCCCGTTGAGGTTATTGGTGAAGTGACT
GCGGATCTTGGCGACTACGCGGATGAGATTAAGGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT192030 NZ_CP069883:c35346-35281 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin