Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3523863..3524425 Replicon chromosome
Accession NZ_CP069882
Organism Escherichia coli strain FDAARGOS_1289

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag I6K19_RS16800 Protein ID WP_000605676.1
Coordinates 3524147..3524425 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6K19_RS16795 Protein ID WP_000781370.1
Coordinates 3523863..3524147 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K19_RS16780 (3519045) 3519045..3522092 + 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -
I6K19_RS16785 (3522105) 3522105..3522989 + 885 WP_001240565.1 formate dehydrogenase N subunit beta -
I6K19_RS16790 (3522982) 3522982..3523635 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
I6K19_RS16795 (3523863) 3523863..3524147 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
I6K19_RS16800 (3524147) 3524147..3524425 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K19_RS16805 (3524611) 3524611..3525621 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
I6K19_RS16810 (3525755) 3525755..3527452 - 1698 WP_024241319.1 malate dehydrogenase -
I6K19_RS16815 (3527609) 3527609..3527746 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6K19_RS16820 (3527848) 3527848..3528063 - 216 WP_000495766.1 biofilm-dependent modulation protein -
I6K19_RS16825 (3528408) 3528408..3528839 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T192016 WP_000605676.1 NZ_CP069882:c3524425-3524147 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T192016 NZ_CP092037:4014557-4014730 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT192016 WP_000781370.1 NZ_CP069882:c3524147-3523863 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT192016 NZ_CP092037:4014233-4014529 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References