Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 364109..364908 Replicon chromosome
Accession NZ_CP069882
Organism Escherichia coli strain FDAARGOS_1289

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag I6K19_RS01785 Protein ID WP_000347269.1
Coordinates 364444..364908 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0YUB5
Locus tag I6K19_RS01780 Protein ID WP_001309780.1
Coordinates 364109..364444 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K19_RS01765 (359894) 359894..360664 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
I6K19_RS01770 (360680) 360680..362014 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
I6K19_RS01775 (362389) 362389..363960 + 1572 WP_001273738.1 galactarate dehydratase -
I6K19_RS01780 (364109) 364109..364444 + 336 WP_001309780.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
I6K19_RS01785 (364444) 364444..364908 + 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
I6K19_RS01790 (364963) 364963..365772 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
I6K19_RS01795 (366021) 366021..367301 + 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
I6K19_RS01800 (367324) 367324..367797 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
I6K19_RS01805 (367808) 367808..368587 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
I6K19_RS01810 (368577) 368577..369455 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
I6K19_RS01815 (369473) 369473..369907 + 435 WP_000948818.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 354867..364908 10041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T192001 WP_000347269.1 NZ_CP069882:364444-364908 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T192001 NZ_CP092037:c1162673-1162566 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12345.94 Da        Isoelectric Point: 4.8616

>AT192001 WP_001309780.1 NZ_CP069882:364109-364444 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT192001 NZ_CP092037:1162720-1162786 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB V0YUB5

References