Detailed information of TA system TA191972

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 4533611..4533982
• Replicon: chromosome
• Accession: NZ_CP069865
• Organism: Escherichia coli strain FDAARGOS_1282

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: I6K12_RS22210
• Protein ID: WP_001443846.1
• Coordinates: 4533833..4533982 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4533611..4533789 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K12_RS25995 (4529363)	4529363..4529536	+	174	WP_001296046.1	protein YnaL	-
I6K12_RS22185 (4529566)	4529566..4530939	+	1374	WP_000123748.1	ATP-dependent RNA helicase DbpA	-
I6K12_RS22190 (4531068)	4531068..4532003	-	936	WP_001523172.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
I6K12_RS22195 (4532055)	4532055..4533290	-	1236	WP_000040839.1	site-specific integrase	-
I6K12_RS22200 (4533292)	4533292..4533507	-	216	WP_000079604.1	excisionase XisR	-
- (4533611)	4533611..4533789	+	179	NuclAT_0	-	Antitoxin
- (4533611)	4533611..4533789	+	179	NuclAT_0	-	Antitoxin
- (4533611)	4533611..4533789	+	179	NuclAT_0	-	Antitoxin
- (4533611)	4533611..4533789	+	179	NuclAT_0	-	Antitoxin
I6K12_RS22205 (4533607)	4533607..4533795	-	189	WP_001302840.1	DUF1187 family protein	-
I6K12_RS22210 (4533833)	4533833..4533982	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
I6K12_RS22215 (4534038)	4534038..4534847	-	810	WP_000166313.1	recombination protein RecT	-
I6K12_RS22220 (4534840)	4534840..4537440	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
I6K12_RS22225 (4537542)	4537542..4537817	-	276	WP_001344816.1	hypothetical protein	-
I6K12_RS22230 (4537892)	4537892..4538062	-	171	WP_001352098.1	YdaE family protein	-
I6K12_RS22235 (4538062)	4538062..4538283	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4527242..4579056	51814

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T191972 WP_001443846.1 NZ_CP069865:c4533982-4533833 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T191972 NZ_CP092012:c1068818-1068291 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCATGGATTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGACACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCGGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT191972 NZ_CP069865:4533611-4533789 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin