Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 3253182..3253876 | Replicon | chromosome |
| Accession | NZ_CP069865 | ||
| Organism | Escherichia coli strain FDAARGOS_1282 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | A0A0D8WHS4 |
| Locus tag | I6K12_RS16050 | Protein ID | WP_001521903.1 |
| Coordinates | 3253478..3253876 (+) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1QAE3 |
| Locus tag | I6K12_RS16045 | Protein ID | WP_000554758.1 |
| Coordinates | 3253182..3253475 (+) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| I6K12_RS16020 (3248297) | 3248297..3249046 | + | 750 | WP_016233951.1 | C40 family peptidase | - |
| I6K12_RS16025 (3249103) | 3249103..3249456 | - | 354 | WP_024166466.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| I6K12_RS16030 (3249535) | 3249535..3251247 | - | 1713 | Protein_3006 | flagellar biosynthesis protein FlhA | - |
| I6K12_RS16035 (3251219) | 3251219..3252004 | + | 786 | WP_000207556.1 | putative lateral flagellar export/assembly protein LafU | - |
| I6K12_RS16040 (3252075) | 3252075..3253130 | + | 1056 | WP_001226177.1 | DNA polymerase IV | - |
| I6K12_RS16045 (3253182) | 3253182..3253475 | + | 294 | WP_000554758.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| I6K12_RS16050 (3253478) | 3253478..3253876 | + | 399 | WP_001521903.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| I6K12_RS16055 (3253886) | 3253886..3254338 | + | 453 | WP_023144376.1 | GNAT family N-acetyltransferase | - |
| I6K12_RS16060 (3254583) | 3254583..3254789 | + | 207 | Protein_3012 | RtcB family protein | - |
| I6K12_RS16065 (3254785) | 3254785..3255137 | + | 353 | Protein_3013 | peptide chain release factor H | - |
| I6K12_RS16070 (3255194) | 3255194..3256651 | - | 1458 | WP_001293009.1 | cytosol nonspecific dipeptidase | - |
| I6K12_RS16075 (3256912) | 3256912..3257370 | + | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| - (3257966) | 3257966..3258046 | + | 81 | NuclAT_11 | - | - |
| - (3257966) | 3257966..3258046 | + | 81 | NuclAT_11 | - | - |
| - (3257966) | 3257966..3258046 | + | 81 | NuclAT_11 | - | - |
| - (3257966) | 3257966..3258046 | + | 81 | NuclAT_11 | - | - |
| I6K12_RS16080 (3257462) | 3257462..3258706 | + | 1245 | WP_000189541.1 | esterase FrsA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15471.88 Da Isoelectric Point: 8.0949
>T191965 WP_001521903.1 NZ_CP069865:3253478-3253876 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T191965 NZ_CP092009:4153340-4153756 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGAGTAAAACCTACATGCTGGATACCTGCATCTGTTCTTTCATTATGCGCGAACAGCCGGAAGCTGTGCTGAAACGCCT
TGAGCAGGCAGTGCTGCGCCGCCATCGTATTGTGGTGTCGGCGATTACCTACGCCGAAATGCGTTTTGGCTGTACGGGCA
AAAAGGCCTCGCCGCGCCATGCCCAACTGGTCGATGCATTCTGTAGTCGCCTGGATGCCGTGCTGGCCTGGGACCGGGCG
GCAGTGGATGCCACCACAGAGATCCGTGCCGTACTGGCCGCCGCCGGTACGCCGATTGGATCAAACGATGCTGCTATCGC
CGGCCATGCCATTGCGTCCGGGGCGATACTGGTCACTAATAACGTGAGGGAGTTTGAGCGGGTACCGGGTTTGCAGTACG
AAGATTGGGTAAAATAA
ATGAGTAAAACCTACATGCTGGATACCTGCATCTGTTCTTTCATTATGCGCGAACAGCCGGAAGCTGTGCTGAAACGCCT
TGAGCAGGCAGTGCTGCGCCGCCATCGTATTGTGGTGTCGGCGATTACCTACGCCGAAATGCGTTTTGGCTGTACGGGCA
AAAAGGCCTCGCCGCGCCATGCCCAACTGGTCGATGCATTCTGTAGTCGCCTGGATGCCGTGCTGGCCTGGGACCGGGCG
GCAGTGGATGCCACCACAGAGATCCGTGCCGTACTGGCCGCCGCCGGTACGCCGATTGGATCAAACGATGCTGCTATCGC
CGGCCATGCCATTGCGTCCGGGGCGATACTGGTCACTAATAACGTGAGGGAGTTTGAGCGGGTACCGGGTTTGCAGTACG
AAGATTGGGTAAAATAA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.76 Da Isoelectric Point: 5.2170
>AT191965 WP_000554758.1 NZ_CP069865:3253182-3253475 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT191965 NZ_CP092009:4153113-4153343 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGCGAACCGTATCTGTATTTAAAAATGGCAATAACCGCGCGATCCGCCTTCCACGCGATCTGGATTTTGACGGCGTCAA
CGAACTTGAAATCTTCCGCGAAGGCGACACGATTATACTGCGCCCCGCCCGCCCTTCCTGGGGATCATTCCGTCATGAGG
AAAAAGCCAGCCCAGACTTTCTGACGGAACGCGAAGACATCATCAGCGATGAGGGACGCTTTGAACTATGA
ATGCGAACCGTATCTGTATTTAAAAATGGCAATAACCGCGCGATCCGCCTTCCACGCGATCTGGATTTTGACGGCGTCAA
CGAACTTGAAATCTTCCGCGAAGGCGACACGATTATACTGCGCCCCGCCCGCCCTTCCTGGGGATCATTCCGTCATGAGG
AAAAAGCCAGCCCAGACTTTCTGACGGAACGCGAAGACATCATCAGCGATGAGGGACGCTTTGAACTATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0D8WHS4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | S1QAE3 |