Detailed information of TA system TA191955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1078286..1079013
• Replicon: chromosome
• Accession: NZ_CP069865
• Organism: Escherichia coli strain FDAARGOS_1282

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: I6K12_RS05805
• Protein ID: WP_001521139.1
• Coordinates: 1078702..1079013 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K12_RS05800
• Protein ID: WP_000126294.1
• Coordinates: 1078286..1078705 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K12_RS05780 (1073371)	1073371..1073898	-	528	WP_001078777.1	electron transport protein HydN	-
I6K12_RS05785 (1074047)	1074047..1075057	-	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
I6K12_RS05790 (1075317)	1075317..1076774	+	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
I6K12_RS05795 (1076783)	1076783..1078207	+	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
I6K12_RS05800 (1078286)	1078286..1078705	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
I6K12_RS05805 (1078702)	1078702..1079013	-	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6K12_RS05810 (1079180)	1079180..1079650	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
I6K12_RS05815 (1079643)	1079643..1080053	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
I6K12_RS05820 (1080050)	1080050..1080817	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
I6K12_RS05825 (1080817)	1080817..1081359	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
I6K12_RS05830 (1081369)	1081369..1083078	-	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T191955 WP_001521139.1 NZ_CP069865:c1079013-1078702 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T191955 NZ_CP092004:c4645455-4645144 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT191955 WP_000126294.1 NZ_CP069865:c1078705-1078286 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT191955 NZ_CP092004:c4645143-4644853 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTAAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGCTCACCGACAGGGCCAGCCAAAGCATTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9