Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4785445..4786042 Replicon chromosome
Accession NZ_CP069853
Organism Klebsiella pneumoniae strain FDAARGOS_1324

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag I6K54_RS23590 Protein ID WP_004142563.1
Coordinates 4785725..4786042 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag I6K54_RS23585 Protein ID WP_004142561.1
Coordinates 4785445..4785732 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K54_RS23555 4781525..4781773 + 249 WP_002893037.1 DUF1158 domain-containing protein -
I6K54_RS23560 4781791..4782132 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
I6K54_RS23565 4782163..4783278 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
I6K54_RS23570 4783458..4784039 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
I6K54_RS23575 4784039..4784407 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
I6K54_RS23580 4784527..4785180 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
I6K54_RS23585 4785445..4785732 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
I6K54_RS23590 4785725..4786042 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6K54_RS23595 4786227..4787270 - 1044 WP_023284821.1 DUF2157 domain-containing protein -
I6K54_RS23600 4787936..4788802 - 867 WP_032436988.1 helix-turn-helix transcriptional regulator -
I6K54_RS23605 4788911..4790338 + 1428 WP_032411778.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T191920 WP_004142563.1 NZ_CP069853:c4786042-4785725 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T191920 NZ_CP091997:4215350-4215661 [Salmonella enterica subsp. enterica serovar Kentucky]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT191920 WP_004142561.1 NZ_CP069853:c4785732-4785445 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT191920 NZ_CP091997:4215658-4216104 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References